| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2007-12-03 |
| タイトル |
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タイトル |
Integrins are not involved in the process of human sperm-oolemmal fusion |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
千石, 一雄
Takuma, N
Miyamoto, T
Horikawa, M
Ishikawa, M
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| 著者 ローマ字 |
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Sengoku, Kazuo |
| 書誌情報 |
Human Reproduction
巻 19,
号 3,
p. 639-644,
発行日 2004-03-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0268-1161 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1093/humrep/deh095 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://humrep.oxfordjournals.org/cgi/reprint/19/3/639 | http://humrep.oxfordjournals.org/cgi/reprint/19/3/639 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
BACKGROUND: We investigated whether integrins are required for the human sperm–oocyte binding and fusion processes. METHODS: The expression of several integrin subunits at the human oocyte plasma membrane was investigated using immunofluorescence microscopy, and the functional role of integrins expressed at the human oocyte surface in sperm–oocyte interaction was studied using a zona-free human oocyte binding and fusion assay. A total of 144 unfertilized oocytes were stained with anti-integrin antibodies and 147 zona-free unfertilized oocytes were inseminated in the presence of various anti-integrin antibodies that were expressed in oocyte plasma membrane. RESULTS: The antibodies of six integrin subunits (2, 3, 5, 6, V, M) and six integrin subunits (1, 2, 3, 4, 5, 6) were bound to the surface of fixed unfertilized oocytes. In contrast, the presence of 1 and 4 subunits could not be verified. The human sperm–oocyte binding was only partially inhibited by blocking antibodies of 2, 3, 5, 6, V, M, 1, 2 and 3 with a maximum of 55% inhibition, but antibodies of 4, 5 and 6 showed no effect on sperm–oolemmal binding. A similar reduction of the number of fused sperm was observed. However, the ratio of fused sperm to total sperm (bound and fused) was not impaired by all integrin antibodies, suggesting that integrins had no role in the sperm–oolemmal fusion process. CONCLUSIONS: These results suggest that one of the binding mechanisms can be inhibited by integrin antibodies but that this mechanism does not play an essential role in the human sperm–oolemmal binding and fusion processes. The other mechanisms, insensitive to integrins, may involve both binding and fusion processes in human oocytes. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Oxford University Press, Human Reproduction, 19(3), 2004, 639-644 is available online at: http://humrep.oxfordjournals.org/cgi/content/abstract/19/3/639.
\nauthor |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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14998963 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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960 |
790.pdf (452.4 kB)
