{"created":"2023-06-19T11:20:34.682403+00:00","id":629,"links":{},"metadata":{"_buckets":{"deposit":"2d5038e3-56cb-421b-81b6-0e50f2a2d891"},"_deposit":{"created_by":3,"id":"629","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"629"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00000629","sets":["7","7:24"]},"author_link":["1472","1473","1474","1475","1476"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"644","bibliographicPageStart":"639","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"Human Reproduction"}]}]},"item_5_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"http://humrep.oxfordjournals.org/cgi/reprint/19/3/639 | http://humrep.oxfordjournals.org/cgi/reprint/19/3/639","subitem_description_type":"Other"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND: We investigated whether integrins are required for the human sperm–oocyte binding and fusion processes. METHODS: The expression of several integrin subunits at the human oocyte plasma membrane was investigated using immunofluorescence microscopy, and the functional role of integrins expressed at the human oocyte surface in sperm–oocyte interaction was studied using a zona-free human oocyte binding and fusion assay. A total of 144 unfertilized oocytes were stained with anti-integrin antibodies and 147 zona-free unfertilized oocytes were inseminated in the presence of various anti-integrin antibodies that were expressed in oocyte plasma membrane. RESULTS: The antibodies of six  integrin subunits (2, 3, 5, 6, V, M) and six  integrin subunits (1, 2, 3, 4, 5, 6) were bound to the surface of fixed unfertilized oocytes. In contrast, the presence of 1 and 4 subunits could not be verified. The human sperm–oocyte binding was only partially inhibited by blocking antibodies of 2, 3, 5, 6, V, M, 1, 2 and 3 with a maximum of 55% inhibition, but antibodies of 4, 5 and 6 showed no effect on sperm–oolemmal binding. A similar reduction of the number of fused sperm was observed. However, the ratio of fused sperm to total sperm (bound and fused) was not impaired by all integrin antibodies, suggesting that integrins had no role in the sperm–oolemmal fusion process. CONCLUSIONS: These results suggest that one of the binding mechanisms can be inhibited by integrin antibodies but that this mechanism does not play an essential role in the human sperm–oolemmal binding and fusion processes. The other mechanisms, insensitive to integrins, may involve both binding and fusion processes in human oocytes.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Oxford University Press, Human Reproduction, 19(3), 2004, 639-644 is available online at: http://humrep.oxfordjournals.org/cgi/content/abstract/19/3/639.\n\\nauthor","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/humrep/deh095","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0268-1161","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"14998963"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"2314"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"960"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Sengoku, Kazuo"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"千石, 一雄"},{"creatorName":"センゴク, カズオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1472","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takuma, N"}],"nameIdentifiers":[{"nameIdentifier":"1473","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyamoto, T"}],"nameIdentifiers":[{"nameIdentifier":"1474","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Horikawa, M"}],"nameIdentifiers":[{"nameIdentifier":"1475","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishikawa, M"}],"nameIdentifiers":[{"nameIdentifier":"1476","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"790.pdf","filesize":[{"value":"452.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"790.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/629/files/790.pdf"},"version_id":"65a13abe-7a7d-4f74-a39e-b60d0a732ec2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Integrins are not involved in the process of human sperm-oolemmal fusion","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Integrins are not involved in the process of human sperm-oolemmal fusion","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["6","7","24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-12-03"},"publish_date":"2007-12-03","publish_status":"0","recid":"629","relation_version_is_last":true,"title":["Integrins are not involved in the process of human sperm-oolemmal fusion"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-19T11:30:06.418956+00:00"}