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Hypothermia-induced activation of the splenic platelet pool as a risk factor for thrombotic disease in a mouse model.
https://asahikawa-med.repo.nii.ac.jp/records/6264
https://asahikawa-med.repo.nii.ac.jp/records/6264e2d73aaf-fa66-45a6-835e-257ff9487087
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
7556.pdf (5.8 MB)
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| Item type | 学位論文 / Thesis or Dissertation_02(1) | |||||||||
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| 公開日 | 2021-04-23 | |||||||||
| タイトル | ||||||||||
| タイトル | Hypothermia-induced activation of the splenic platelet pool as a risk factor for thrombotic disease in a mouse model. | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | hypothermia | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | microthrombosis | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | platelet activation | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | spleen | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | von Willebrand factor | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| その他（別言語等）のタイトル | ||||||||||
| その他のタイトル | マウス低体温症モデルにおける脾臓内血小板活性化は血栓症の危険因子となる | |||||||||
| 言語 | ja | |||||||||
| 著者 |
堀岡, 希衣
× 堀岡, 希衣
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| 著者 ローマ字 | ||||||||||
| Horioka, Kie | ||||||||||
| 書誌情報 |
Journal of thrombosis and haemostasis 巻 17, 号 10, p. 1762-1771, 発行日 2019-10-01 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 1538-7933 | |||||||||
| DOI | ||||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1111/jth.14555 | |||||||||
| 識別番号 その他 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | PMID: 31237986 | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第547号 | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2020-03-25 | |||||||||
| 学位名 | ||||||||||
| 言語 | ja | |||||||||
| 学位名 | 博士（医学） | |||||||||
| 学位授与機関 | ||||||||||
| 言語 | ja | |||||||||
| 学位授与機関名 | 旭川医科大学 | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Background: Hypothermia, either therapeutically induced or accidental (ie, an involuntary decrease in core body temperature to <35°C), results in hemostatic disorders. However, it remains unclear whether hypothermia enhances or inhibits coagulation, especially in severe hypothermia. The present study evaluated the thrombocytic and hemostatic changes in hypothermic mice. \nMethods: C57Bl/6 mice were placed at an ambient temperature of -20°C under general anesthesia. When the rectal temperature decreased to 15°C, 10 mice were immediately euthanized, while another 10 mice were rewarmed, kept in normal conditions for 24 hours, and then euthanized. These treatments were also performed in 20 splenectomized mice. \nResults: The hypothermic mice had adhesion of CD62P-positive platelets with high expression of von Willebrand factor (vWF) in their spleens, while the status of the peripheral platelets was unchanged. Furthermore, the plasma levels of platelet factor 4 (PF4) and pro-platelet basic protein (PPBP), which are biomarkers for platelet degranulation, were significantly higher in hypothermic mice than in control mice, indicating that hypothermia activated the platelets in the splenic pool. Thus, we analyzed these biomarkers in asplenic mice. There was no increase in either PF4 or PPBP in splenectomized hypothermic mice. Additionally, the plasma D-dimer elevation and microthrombosis were caused in rewarmed mice, but not in asplenic rewarmed mice. \nConclusions: Our results indicate that hypothermia leads to platelet activation in the spleen via the upregulation of vWF, and this activation causes hypercoagulability after rewarming. |
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| 資源タイプ | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| フォーマット | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
