{"created":"2023-06-19T11:25:24.707637+00:00","id":6264,"links":{},"metadata":{"_buckets":{"deposit":"f7b96a27-b1ef-44cb-9da0-459ed35185f9"},"_deposit":{"created_by":3,"id":"6264","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"6264"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00006264","sets":["7","7:27:45"]},"author_link":["19662"],"control_number":"6264","item_10_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"マウス低体温症モデルにおける脾臓内血小板活性化は血栓症の危険因子となる","subitem_alternative_title_language":"ja"}]},"item_10_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-10-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"1771","bibliographicPageStart":"1762","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"Journal of thrombosis and haemostasis"}]}]},"item_10_date_granted_30":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2020-03-25"}]},"item_10_degree_grantor_32":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"旭川医科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"10107","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10_degree_name_31":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_10_description_28":{"attribute_name":"識別番号 その他","attribute_value_mlt":[{"subitem_description":"PMID: 31237986","subitem_description_type":"Other"}]},"item_10_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Hypothermia, either therapeutically induced or accidental (ie, an involuntary decrease in core body temperature to <35°C), results in hemostatic disorders. However, it remains unclear whether hypothermia enhances or inhibits coagulation, especially in severe hypothermia. The present study evaluated the thrombocytic and hemostatic changes in hypothermic mice.\n\\nMethods: C57Bl/6 mice were placed at an ambient temperature of -20°C under general anesthesia. When the rectal temperature decreased to 15°C, 10 mice were immediately euthanized, while another 10 mice were rewarmed, kept in normal conditions for 24 hours, and then euthanized. These treatments were also performed in 20 splenectomized mice.\n\\nResults: The hypothermic mice had adhesion of CD62P-positive platelets with high expression of von Willebrand factor (vWF) in their spleens, while the status of the peripheral platelets was unchanged. Furthermore, the plasma levels of platelet factor 4 (PF4) and pro-platelet basic protein (PPBP), which are biomarkers for platelet degranulation, were significantly higher in hypothermic mice than in control mice, indicating that hypothermia activated the platelets in the splenic pool. Thus, we analyzed these biomarkers in asplenic mice. There was no increase in either PF4 or PPBP in splenectomized hypothermic mice. Additionally, the plasma D-dimer elevation and microthrombosis were caused in rewarmed mice, but not in asplenic rewarmed mice.\n\\nConclusions: Our results indicate that hypothermia leads to platelet activation in the spleen via the upregulation of vWF, and this activation causes hypercoagulability after rewarming.","subitem_description_type":"Abstract"}]},"item_10_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_dissertation_number_29":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第547号"}]},"item_10_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/jth.14555","subitem_relation_type_select":"DOI"}}]},"item_10_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1538-7933","subitem_source_identifier_type":"ISSN"}]},"item_10_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Horioka, Kie"}]},"item_10_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"堀岡, 希衣","creatorNameLang":"ja"},{"creatorName":"ホリオカ, キエ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"19662","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"7556.pdf","filesize":[{"value":"5.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"7556.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/6264/files/7556.pdf"},"version_id":"d541099d-1b56-4804-853a-4a10afd06cbb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"hypothermia","subitem_subject_scheme":"Other"},{"subitem_subject":"microthrombosis","subitem_subject_scheme":"Other"},{"subitem_subject":"platelet activation","subitem_subject_scheme":"Other"},{"subitem_subject":"spleen","subitem_subject_scheme":"Other"},{"subitem_subject":"von Willebrand factor","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Hypothermia-induced activation of the splenic platelet pool as a risk factor for thrombotic disease in a mouse model.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hypothermia-induced activation of the splenic platelet pool as a risk factor for thrombotic disease in a mouse model.","subitem_title_language":"en"}]},"item_type_id":"10","owner":"3","path":["6","7","45"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-04-23"},"publish_date":"2021-04-23","publish_status":"0","recid":"6264","relation_version_is_last":true,"title":["Hypothermia-induced activation of the splenic platelet pool as a risk factor for thrombotic disease in a mouse model."],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-10T11:23:06.943451+00:00"}