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Activation of muscarinic receptors prevents TNF-α-mediated intestinal epithelial barrier disruption through p38 MAPK.
https://asahikawa-med.repo.nii.ac.jp/records/5925
https://asahikawa-med.repo.nii.ac.jp/records/5925d9207f26-a493-432e-a3f5-6d93d5d6f7f6
名前 / ファイル | ライセンス | アクション |
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6801.pdf (12.8 MB)
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Item type | 学術雑誌論文 / Journal Article_02(1) | |||||
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公開日 | 2017-09-22 | |||||
タイトル | ||||||
タイトル | Activation of muscarinic receptors prevents TNF-α-mediated intestinal epithelial barrier disruption through p38 MAPK. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
キーワード | Inflammatory bowel disease | |||||
キーワード | ||||||
主題Scheme | Other | |||||
キーワード | Tumor necrosis factor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
キーワード | Intestinal barrier | |||||
キーワード | ||||||
主題Scheme | Other | |||||
キーワード | Muscarinic acetylcholine receptor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
キーワード | Histamine receptor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
キーワード | p38 MAPK | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
著者 |
宇和田, 淳介
× 宇和田, 淳介× 矢澤, 隆志× Md, Tariqul Islam× Md, Rafiqul IslamKhan× Susanne, M.Krug× Michael, Fromm× 唐木, 晋一郎× 鈴木, 裕一× 桑原, 厚和× 吉木, はつみ× 定, 清直× 村松, 郁延× 谷口, 隆信 |
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著者 ローマ字 | ||||||
Uwada, Junsuke | ||||||
著者 ローマ字 | ||||||
Yazawa, Takashi | ||||||
著者 ローマ字 | ||||||
著者 ローマ字 | ||||||
著者 ローマ字 | ||||||
著者 ローマ字 | ||||||
著者 ローマ字 | ||||||
Karaki, Shin-ichiro | ||||||
著者 ローマ字 | ||||||
Suzuki, Yuichi | ||||||
著者 ローマ字 | ||||||
Kuwahara, Atsukazu | ||||||
著者 ローマ字 | ||||||
Yoshiki, Hatsumi | ||||||
著者 ローマ字 | ||||||
Sada, Kiyonao | ||||||
著者 ローマ字 | ||||||
Muramatsu, Ikunobu | ||||||
著者 ローマ字 | ||||||
Taniguchi, Takanobu | ||||||
書誌情報 |
Cellular Signalling 巻 35, p. 188-196, 発行日 2017-07-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0898-6568 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.cellsig.2017.04.007 | |||||
リンクURL | ||||||
内容記述タイプ | Other | |||||
内容記述 | http://www.sciencedirect.com/science/article/pii/S0898656817301079?via%3Dihub | http://www.sciencedirect.com/science/article/pii/S0898656817301079?via%3Dihub | |||||
識別番号 その他 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PMID:28412413 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Intestinal epithelial cells form a tight barrier to act as selective physical barriers, repelling hostile substances. Tumor necrosis factor-α (TNF-α) is a well characterized pro-inflammatory cytokine which can compromise intestinal barrier function and the suppression of TNF-α function is important for treatment of inflammatory bowel disease (IBD). In this study, we investigated the contribution of G-protein-coupled receptor (GPCR)-induced signalling pathways to the maintenance of epithelial barrier function. We first demonstrated the existence of functional muscarinic M3 and histamine H1 receptors in colonic epithelial cell HT-29/B6. As we previously reported, muscarinic M3 receptor prevented TNF-α-induced barrier disruption through acceleration of TNF receptor (TNFR) shedding which is carried out by TNF-α converting enzyme (TACE). M3 receptor-mediated suppression of TNF-α function depends on Gαq/11 protein, however, histamine H1 receptor could not ameliorate TNF-α function, while which could induce Gαq/11 dependent intracellular Ca2+ mobilization. We found that p38 MAPK was predominantly phosphorylated by M3 receptor through Gαq/11 protein, whereas H1 receptor barely upregulated the phosphorylation. Inhibition of p38 MAPK abolished M3 receptor-mediated TNFR shedding and suppression of TNF-α-induced NF-κB signalling. The p38 MAPK was also involved in TACE- mediated EGFR transactivation followed by ERK1/2 phosphorylation. These results indicate that not H1 but M3 receptor-induced activation of p38 MAPK might contribute to the maintenance of epithelial barrier function through down-regulation of TNF-α signalling and activation of EGFR. | |||||
注記 | ||||||
内容記述タイプ | Other | |||||
注記 | CC-BY-NC-ND | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
資源タイプ | text | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
ID(XooNIps) | ||||||
28412413 | ||||||
閲覧数(XooNIps) | ||||||
461 | ||||||
ダウンロード数(XooNIps) | ||||||
422 |