{"created":"2023-06-19T11:25:06.323344+00:00","id":5925,"links":{},"metadata":{"_buckets":{"deposit":"72c741cc-ab99-4574-b77b-146e6f1ee2fb"},"_deposit":{"created_by":1,"id":"5925","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"5925"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00005925","sets":["7","7:24"]},"author_link":["18629","18634","18636","18640","18633","18639","18631","18635","18637","18641","18638","18630","18632"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-07-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"196","bibliographicPageStart":"188","bibliographicVolumeNumber":"35","bibliographic_titles":[{"bibliographic_title":"Cellular Signalling"}]}]},"item_5_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"http://www.sciencedirect.com/science/article/pii/S0898656817301079?via%3Dihub | http://www.sciencedirect.com/science/article/pii/S0898656817301079?via%3Dihub","subitem_description_type":"Other"}]},"item_5_description_28":{"attribute_name":"識別番号 その他","attribute_value_mlt":[{"subitem_description":"PMID:28412413","subitem_description_type":"Other"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Intestinal epithelial cells form a tight barrier to act as selective physical barriers, repelling hostile substances. Tumor necrosis factor-α (TNF-α) is a well characterized pro-inflammatory cytokine which can compromise intestinal barrier function and the suppression of TNF-α function is important for treatment of inflammatory bowel disease (IBD). In this study, we investigated the contribution of G-protein-coupled receptor (GPCR)-induced signalling pathways to the maintenance of epithelial barrier function. We first demonstrated the existence of functional muscarinic M3 and histamine H1 receptors in colonic epithelial cell HT-29/B6. As we previously reported, muscarinic M3 receptor prevented TNF-α-induced barrier disruption through acceleration of TNF receptor (TNFR) shedding which is carried out by TNF-α converting enzyme (TACE). M3 receptor-mediated suppression of TNF-α function depends on Gαq/11 protein, however, histamine H1 receptor could not ameliorate TNF-α function, while which could induce Gαq/11 dependent intracellular Ca2+ mobilization. We found that p38 MAPK was predominantly phosphorylated by M3 receptor through Gαq/11 protein, whereas H1 receptor barely upregulated the phosphorylation. Inhibition of p38 MAPK abolished M3 receptor-mediated TNFR shedding and suppression of TNF-α-induced NF-κB signalling. The p38 MAPK was also involved in TACE- mediated EGFR transactivation followed by ERK1/2 phosphorylation. These results indicate that not H1 but M3 receptor-induced activation of p38 MAPK might contribute to the maintenance of epithelial barrier function through down-regulation of TNF-α signalling and activation of EGFR.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"CC-BY-NC-ND","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.cellsig.2017.04.007","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0898-6568","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"28412413"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"461"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"422"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Uwada, Junsuke"},{"subitem_text_value":"Yazawa, Takashi"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"Karaki, Shin-ichiro"},{"subitem_text_value":"Suzuki, Yuichi"},{"subitem_text_value":"Kuwahara, Atsukazu"},{"subitem_text_value":"Yoshiki, Hatsumi"},{"subitem_text_value":"Sada, Kiyonao"},{"subitem_text_value":"Muramatsu, Ikunobu"},{"subitem_text_value":"Taniguchi, Takanobu"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"宇和田, 淳介"},{"creatorName":"ウワダ, ジュンスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"18629","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"矢澤, 隆志"},{"creatorName":"ヤザワ, タカシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"18630","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Md, Tariqul Islam"}],"nameIdentifiers":[{"nameIdentifier":"18631","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Md, Rafiqul IslamKhan"}],"nameIdentifiers":[{"nameIdentifier":"18632","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Susanne, M.Krug"}],"nameIdentifiers":[{"nameIdentifier":"18633","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Michael, Fromm"}],"nameIdentifiers":[{"nameIdentifier":"18634","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"唐木, 晋一郎"},{"creatorName":"カラキ, シンイチロウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"18635","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木, 裕一"},{"creatorName":"スズキ, ユウイチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"18636","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"桑原, 厚和"},{"creatorName":"クワハラ, アツカズ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"18637","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉木, はつみ"},{"creatorName":"ヨシキ, ハツミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"18638","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"定, 清直"},{"creatorName":"サダ, キヨナオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"18639","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"村松, 郁延"},{"creatorName":"ムラマツ, イクノブ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"18640","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"谷口, 隆信"},{"creatorName":"タニグチ, タカノzブ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"18641","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"6801.pdf","filesize":[{"value":"12.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"6801.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/5925/files/6801.pdf"},"version_id":"e352f29e-b063-411a-a041-249ac6242ce1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Inflammatory bowel disease","subitem_subject_scheme":"Other"},{"subitem_subject":"Tumor necrosis factor","subitem_subject_scheme":"Other"},{"subitem_subject":"Intestinal barrier","subitem_subject_scheme":"Other"},{"subitem_subject":"Muscarinic acetylcholine receptor","subitem_subject_scheme":"Other"},{"subitem_subject":"Histamine receptor","subitem_subject_scheme":"Other"},{"subitem_subject":"p38 MAPK","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Activation of muscarinic receptors prevents TNF-α-mediated intestinal epithelial barrier disruption through p38 MAPK.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Activation of muscarinic receptors prevents TNF-α-mediated intestinal epithelial barrier disruption through p38 MAPK."}]},"item_type_id":"5","owner":"1","path":["6","7","24"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-09-22"},"publish_date":"2017-09-22","publish_status":"0","recid":"5925","relation_version_is_last":true,"title":["Activation of muscarinic receptors prevents TNF-α-mediated intestinal epithelial barrier disruption through p38 MAPK."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-19T12:08:07.474360+00:00"}