| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2017-09-27 |
| タイトル |
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タイトル |
11-Ketotestosterone Is a Major Androgen Produced in Human Gonads |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
今道, 力敬
結城, 幸一
折坂, 誠
北野, 健
向井, 邦晃
牛首, 文隆
谷口, 隆信
梅澤, 明弘
宮本, 薫
矢澤, 隆志
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| 著者 ローマ字 |
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Imamichi, Yoshitaka |
| 著者 ローマ字 |
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Yuki, Koh-ichi |
| 著者 ローマ字 |
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Orisaka, Makoto |
| 著者 ローマ字 |
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Kitano, Takeshi |
| 著者 ローマ字 |
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Mukai, Kuniaki |
| 著者 ローマ字 |
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Ushikubi, Fumitaka |
| 著者 ローマ字 |
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Taniguchi, Takanobu |
| 著者 ローマ字 |
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Umezawa, Akihiro |
| 著者 ローマ字 |
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Miyamoto, Kaoru |
| 著者 ローマ字 |
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Yazawa, Takashi |
| 書誌情報 |
The Journal of Clinical Endocrinology and Metabolism
巻 101,
号 10,
p. 3582-3591,
発行日 2016-07-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0021-972X |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1210/jc.2016-2311 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
https://academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2016-2311 | https://academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2016-2311 |
| 識別番号 その他 |
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内容記述タイプ |
Other |
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内容記述 |
PMID：27428878 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
CONTEXT:
\n11-ketotestosterone (11-KT) is a novel class of active androgen. However, the detail of its synthesis remains unknown for humans.
OBJECTIVE:
\nThe objective of this study was to clarify the production and properties of 11-KT in human. Design, Participants, and Methods: Expression of cytochrome P450 and 11β-hydroxysteroid dehydrogenase types 1 and 2 (key enzymes involved in the synthesis of 11-KT) were investigated in human gonads. The production of 11-KT was investigated in Leydig cells. Plasma concentrations of testosterone and 11-KT were measured in 10 women and 10 men of reproductive age. Investigation of its properties was performed using breast cancer-derived MCF-7 cells.
RESULTS:
\nCytochrome P450 and 11β-hydroxysteroid dehydrogenase types 1 and 2 were detected in Leydig cells and theca cells. Leydig cells produced 11-KT, and relatively high levels of plasma 11-KT were measured in both men and women. There was no sexual dimorphism in the plasma levels of 11-KT, even though testosterone levels were more than 20 times higher in men than in women. It is noteworthy that the levels of testosterone and 11-KT were similar in women. In a luciferase reporter system, 11-KT activated human androgen receptor-mediated transactivation. Conversely, 11-KT did not activate estrogen receptor-mediated transactivation in aromatase-expressed MCF-7 cells, whereas testosterone did following conversion to estrogen. 11-KT did not affect the estrogen/estrogen receptor -mediated cell proliferation of MCF-7 cells. Furthermore, it significantly inhibited cell proliferation when androgen receptor was transfected into MCF-7 cells.
CONCLUSIONS:
\nThe current study indicates that 11-KT is produced in the gonads and represents a major androgen in human. It can potentially serve as a nonaromatizable androgen. |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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27428878 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1124 |
6805.pdf (691.3 kB)
