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アイテム
Apoptosis-Resistant Cardiac Progenitor Cells Modified With Apurinic/Apyrimidinic Endonuclease/Redox Factor 1 Gene Overexpression Regulate Cardiac Repair After Myocardial Infarction.
https://asahikawa-med.repo.nii.ac.jp/records/5666
https://asahikawa-med.repo.nii.ac.jp/records/56660980288b-8ef8-401e-b174-affa6dd0aea2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
6720.pdf (2.8 MB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-05-24 | |||||
| タイトル | ||||||
| タイトル | Apoptosis-Resistant Cardiac Progenitor Cells Modified With Apurinic/Apyrimidinic Endonuclease/Redox Factor 1 Gene Overexpression Regulate Cardiac Repair After Myocardial Infarction. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Apoptosis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cellular therapy | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Gene expression | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Sca-1 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Stem/progenitor cell | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
青沼, 達也
× 青沼, 達也× 竹原, 有史× 丸山, 啓介× 鹿原, 真樹× 松木, 考樹× 山内, 敦司× 川辺, 淳一× 長谷部, 直幸 |
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| 著者 ローマ字 | ||||||
| 値 | Aonuma, Tatsuya | |||||
| 著者 ローマ字 | ||||||
| 値 | Takehara, Naofumi | |||||
| 著者 ローマ字 | ||||||
| 値 | Maruyama, Keisuke | |||||
| 著者 ローマ字 | ||||||
| 値 | Kabara, Maki | |||||
| 著者 ローマ字 | ||||||
| 値 | Matsuki, Motoki | |||||
| 著者 ローマ字 | ||||||
| 値 | Yamauchi, Atsushi | |||||
| 著者 ローマ字 | ||||||
| 値 | Kawabe, Jun-ichi | |||||
| 著者 ローマ字 | ||||||
| 値 | Hasebe, Naoyuki | |||||
| 書誌情報 |
Stem cells translational medicine. 巻 5, 号 8, p. 1067-1078, 発行日 2016-08-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2157-6564 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.5966/sctm.2015-0281 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Overcoming the insufficient survival of cell grafts is an essential objective in cell-based therapy. Apurinic/apyrimidinic endonuclease/redox factor 1 (APE1) promotes cell survival and may enhance the therapeutic effect of engrafted cells. The aim of this study is to determine whether APE1 overexpression in cardiac progenitor cells (CPCs) could ameliorate the efficiency of cell-based therapy. CPCs isolated from 8- to 10-week-old C57BL/6 mouse hearts were infected with retrovirus harboring APE1-DsRed (APE1-CPC) or a DsRed control (control-CPC). Oxidative stress-induced apoptosis was then assessed in APE1-CPCs, control-CPCs, and neonatal rat ventricular myocytes (NRVMs) cocultured with these CPCs. This analysis revealed that APE1 overexpression inhibited CPC apoptosis with activation of transforming growth factor β-activated kinase 1 (TAK1) and nuclear factor (NF)-κB. In the coculture model, NRVM apoptosis was inhibited to a greater extent in the presence of APE1-CPCs compared with control-CPCs. Moreover, the number of surviving DsRed-positive CPC grafts was significantly higher 7 days after the transplant of APE1-CPCs into a mouse myocardial infarction model, and the left ventricular ejection fraction showed greater improvement with attenuation of fibrosis 28 days after the transplant of APE1-CPCs compared with control-CPCs. Additionally, fewer inflammatory macrophages and a higher percentage of cardiac α-sarcomeric actinin-positive CPC-grafts were observed in mice injected with APE1-CPCs compared with control-CPCs after 7 days. In conclusion, antiapoptotic APE1-CPC graft, which increased TAK1-NF-κB pathway activation, survived effectively in the ischemic heart, restored cardiac function, and reduced cardiac inflammation and fibrosis. APE1 overexpression in CPCs may serve as a novel strategy to improve cardiac cell therapy. | |||||
| 注記 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | OA誌 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | text | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| ID(XooNIps) | ||||||
| 値 | 27334489 | |||||
| 閲覧数(XooNIps) | ||||||
| 値 | 642 | |||||
| ダウンロード数(XooNIps) | ||||||
| 値 | 844 | |||||
