{"created":"2023-06-19T11:24:52.875219+00:00","id":5666,"links":{},"metadata":{"_buckets":{"deposit":"a29f85f9-87ab-4c0d-8fb7-bc2d052603fa"},"_deposit":{"created_by":1,"id":"5666","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"5666"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00005666","sets":["7","7:24"]},"author_link":["17687","17680","17681","17685","17684","17683","17682","17686"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-08-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"1078","bibliographicPageStart":"1067","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"Stem cells translational medicine."}]}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Overcoming the insufficient survival of cell grafts is an essential objective in cell-based therapy. Apurinic/apyrimidinic endonuclease/redox factor 1 (APE1) promotes cell survival and may enhance the therapeutic effect of engrafted cells. The aim of this study is to determine whether APE1 overexpression in cardiac progenitor cells (CPCs) could ameliorate the efficiency of cell-based therapy. CPCs isolated from 8- to 10-week-old C57BL/6 mouse hearts were infected with retrovirus harboring APE1-DsRed (APE1-CPC) or a DsRed control (control-CPC). Oxidative stress-induced apoptosis was then assessed in APE1-CPCs, control-CPCs, and neonatal rat ventricular myocytes (NRVMs) cocultured with these CPCs. This analysis revealed that APE1 overexpression inhibited CPC apoptosis with activation of transforming growth factor β-activated kinase 1 (TAK1) and nuclear factor (NF)-κB. In the coculture model, NRVM apoptosis was inhibited to a greater extent in the presence of APE1-CPCs compared with control-CPCs. Moreover, the number of surviving DsRed-positive CPC grafts was significantly higher 7 days after the transplant of APE1-CPCs into a mouse myocardial infarction model, and the left ventricular ejection fraction showed greater improvement with attenuation of fibrosis 28 days after the transplant of APE1-CPCs compared with control-CPCs. Additionally, fewer inflammatory macrophages and a higher percentage of cardiac α-sarcomeric actinin-positive CPC-grafts were observed in mice injected with APE1-CPCs compared with control-CPCs after 7 days. In conclusion, antiapoptotic APE1-CPC graft, which increased TAK1-NF-κB pathway activation, survived effectively in the ischemic heart, restored cardiac function, and reduced cardiac inflammation and fibrosis. APE1 overexpression in CPCs may serve as a novel strategy to improve cardiac cell therapy.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"OA誌","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.5966/sctm.2015-0281","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2157-6564","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"27334489"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"642"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"844"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Aonuma, Tatsuya"},{"subitem_text_value":"Takehara, Naofumi"},{"subitem_text_value":"Maruyama, Keisuke"},{"subitem_text_value":"Kabara, Maki"},{"subitem_text_value":"Matsuki, Motoki"},{"subitem_text_value":"Yamauchi, Atsushi"},{"subitem_text_value":"Kawabe, Jun-ichi"},{"subitem_text_value":"Hasebe, Naoyuki"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"青沼, 達也"},{"creatorName":"アオヌマ, タツヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17680","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"竹原, 有史"},{"creatorName":"タケハラ, ナオフミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17681","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"丸山, 啓介"},{"creatorName":"マルヤマ, ケイスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17682","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鹿原, 真樹"},{"creatorName":"カバラ, マキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17683","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松木, 考樹"},{"creatorName":"マツキ, モトキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17684","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山内, 敦司"},{"creatorName":"ヤマウチ, アツシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17685","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"川辺, 淳一"},{"creatorName":"カワベ, ジュンイチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17686","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"長谷部, 直幸"},{"creatorName":"ハセベ, ナオユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17687","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"6720.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"6720.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/5666/files/6720.pdf"},"version_id":"641d5c03-1bb2-4311-99a0-30b759fed7b3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Apoptosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Cellular therapy","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene expression","subitem_subject_scheme":"Other"},{"subitem_subject":"Sca-1","subitem_subject_scheme":"Other"},{"subitem_subject":"Stem/progenitor cell","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Apoptosis-Resistant Cardiac Progenitor Cells Modified With Apurinic/Apyrimidinic Endonuclease/Redox Factor 1 Gene Overexpression Regulate Cardiac Repair After Myocardial Infarction.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Apoptosis-Resistant Cardiac Progenitor Cells Modified With Apurinic/Apyrimidinic Endonuclease/Redox Factor 1 Gene Overexpression Regulate Cardiac Repair After Myocardial Infarction."}]},"item_type_id":"5","owner":"1","path":["6","7","24"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-05-24"},"publish_date":"2017-05-24","publish_status":"0","recid":"5666","relation_version_is_last":true,"title":["Apoptosis-Resistant Cardiac Progenitor Cells Modified With Apurinic/Apyrimidinic Endonuclease/Redox Factor 1 Gene Overexpression Regulate Cardiac Repair After Myocardial Infarction."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-19T12:15:51.745398+00:00"}