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網膜虚血再灌流障害における分散ヘスペレチンの神経保護効果
https://asahikawa-med.repo.nii.ac.jp/records/5537
https://asahikawa-med.repo.nii.ac.jp/records/55373512e1c5-42a6-4c6d-a989-cbb3122a2854
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
6396.pdf (2.1 MB)
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| Item type | 学位論文 / Thesis or Dissertation_02(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-04-25 | |||||
| タイトル | ||||||
| タイトル | 網膜虚血再灌流障害における分散ヘスペレチンの神経保護効果 | |||||
| 言語 | ja | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Hesperetin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ischemia–reperfusion | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Neuroprotection | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Retina | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Inflammation | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| タイトル ローマ字 | ||||||
| その他のタイトル | Neuroprotective effect of water-dispersible hesperetin in retinal ischemia reperfusion injury | |||||
| 著者 |
下内, 昭人
× 下内, 昭人 |
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| 著者 ローマ字 | ||||||
| 値 | Shimouchi, Akito | |||||
| 書誌情報 |
Japanese Journal of Ophthalmology 巻 60, 号 1, p. 51-61, 発行日 2016-01-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0021-5155 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1007/s10384-015-0415-z | |||||
| 識別番号 その他 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | PMID:26407617 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲492 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2016-03-25 | |||||
| 学位名 | ||||||
| 学位名 | 博士（医学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 10107 | |||||
| 学位授与機関名 | 旭川医科大学 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose \nTo determine whether water-dispersible hesperetin (WD-Hpt) can prevent degeneration of ganglion cell neurons in the ischemic retina. Methods \nIschemia reperfusion (I/R) injury was induced by increasing the intraocular pressure of mice to 110 mmHg for 40 min. Mice received daily intraperitoneal injections with either normal saline (NS, 0.3 ml/day) or WD-Hpt (0.3 ml, 200 mg/kg/day). Reactive oxygen species (ROS) was assessed by dihydroethidium and nitrotyrosine formation. Inflammation was estimated by microglial morphology in the retina. Lipopolysaccharide (LPS)-stimulated BV-2 cells were used to explore the anti-inflammatory effect of WD-Hpt on activated microglia by quantifying the expression of IL-1β using real-time quantitative reverse transcription-polymerase chain reaction. Ganglion cell loss was assessed by immunohistochemistry of NeuN. Glial activation was quantified with glial fibrillary acidic protein (GFAP) immunoreactivity. Apoptosis was evaluated with a terminal deoxynucleotidyl transferase (TUNEL) assay and immunohistochemistry of cleaved caspase-3. Phosphorylation of extracellular signal-regulated kinase (p-ERK) was surveyed by western blotting. Results \nWD-Hpt decreased I/R-induced ROS formation. WD-Hpt alleviated microglial activation induced by I/R and reduced mRNA levels of IL-1β in LPS-stimulated BV-2. I/R resulted in a 37 % reduction in the number of ganglion cells in the NS-treated mice, whereas the reduction was only 5 % in the WD-Hpt-treated mice. In addition, WD-Hpt mitigated the immunoreactivity of GFAP, increased expression of cleaved caspase-3, increased number of TUNEL positive cells and p-ERK after I/R. Conclusions \nWD-Hpt protected ganglion cells from I/R injury by inhibiting oxidative stress and modulating cell death signaling. Moreover, WD-Hpt had an anti-inflammatory effect through the suppression of activated microglia. |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | First online: 25 September 2015 \nThe final publication is available at Springer via http://dx.doi.org/10.1007/s10384-015-0415-z |
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| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
