{"created":"2023-06-19T11:24:46.364227+00:00","id":5537,"links":{},"metadata":{"_buckets":{"deposit":"62c6968e-02ff-4e29-a84e-c56bd2abe83d"},"_deposit":{"created_by":3,"id":"5537","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5537"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00005537","sets":["7","7:27:41"]},"author_link":["17354"],"item_10_alternative_title_2":{"attribute_name":"タイトル ローマ字","attribute_value_mlt":[{"subitem_alternative_title":"Neuroprotective effect of water-dispersible hesperetin in retinal ischemia reperfusion injury"}]},"item_10_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"61","bibliographicPageStart":"51","bibliographicVolumeNumber":"60","bibliographic_titles":[{"bibliographic_title":"Japanese Journal of Ophthalmology"}]}]},"item_10_date_granted_30":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2016-03-25"}]},"item_10_degree_grantor_32":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"旭川医科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"10107","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10_degree_name_31":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）"}]},"item_10_description_28":{"attribute_name":"識別番号 その他","attribute_value_mlt":[{"subitem_description":"PMID:26407617","subitem_description_type":"Other"}]},"item_10_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose\n\\nTo determine whether water-dispersible hesperetin (WD-Hpt) can prevent degeneration of ganglion cell neurons in the ischemic retina.\nMethods\n\\nIschemia reperfusion (I/R) injury was induced by increasing the intraocular pressure of mice to 110 mmHg for 40 min. Mice received daily intraperitoneal injections with either normal saline (NS, 0.3 ml/day) or WD-Hpt (0.3 ml, 200 mg/kg/day). Reactive oxygen species (ROS) was assessed by dihydroethidium and nitrotyrosine formation. Inflammation was estimated by microglial morphology in the retina. Lipopolysaccharide (LPS)-stimulated BV-2 cells were used to explore the anti-inflammatory effect of WD-Hpt on activated microglia by quantifying the expression of IL-1β using real-time quantitative reverse transcription-polymerase chain reaction. Ganglion cell loss was assessed by immunohistochemistry of NeuN. Glial activation was quantified with glial fibrillary acidic protein (GFAP) immunoreactivity. Apoptosis was evaluated with a terminal deoxynucleotidyl transferase (TUNEL) assay and immunohistochemistry of cleaved caspase-3. Phosphorylation of extracellular signal-regulated kinase (p-ERK) was surveyed by western blotting.\nResults\n\\nWD-Hpt decreased I/R-induced ROS formation. WD-Hpt alleviated microglial activation induced by I/R and reduced mRNA levels of IL-1β in LPS-stimulated BV-2. I/R resulted in a 37 % reduction in the number of ganglion cells in the NS-treated mice, whereas the reduction was only 5 % in the WD-Hpt-treated mice. In addition, WD-Hpt mitigated the immunoreactivity of GFAP, increased expression of cleaved caspase-3, increased number of TUNEL positive cells and p-ERK after I/R.\nConclusions\n\\nWD-Hpt protected ganglion cells from I/R injury by inhibiting oxidative stress and modulating cell death signaling. Moreover, WD-Hpt had an anti-inflammatory effect through the suppression of activated microglia.","subitem_description_type":"Abstract"}]},"item_10_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"First online: 25 September 2015\n\\nThe final publication is available at Springer via http://dx.doi.org/10.1007/s10384-015-0415-z","subitem_description_type":"Other"}]},"item_10_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10_dissertation_number_29":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲492"}]},"item_10_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s10384-015-0415-z","subitem_relation_type_select":"DOI"}}]},"item_10_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-5155","subitem_source_identifier_type":"ISSN"}]},"item_10_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Shimouchi, Akito"}]},"item_10_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"下内, 昭人","creatorNameLang":"ja"},{"creatorName":"シモウチ, アキト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"17354","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"6396.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"6396.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/5537/files/6396.pdf"},"version_id":"8e801e00-ed13-4894-94f2-214b69a2a423"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hesperetin","subitem_subject_scheme":"Other"},{"subitem_subject":"Ischemia–reperfusion","subitem_subject_scheme":"Other"},{"subitem_subject":"Neuroprotection","subitem_subject_scheme":"Other"},{"subitem_subject":"Retina","subitem_subject_scheme":"Other"},{"subitem_subject":"Inflammation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"網膜虚血再灌流障害における分散ヘスペレチンの神経保護効果","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"網膜虚血再灌流障害における分散ヘスペレチンの神経保護効果","subitem_title_language":"ja"}]},"item_type_id":"10","owner":"3","path":["6","7","41"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-04-25"},"publish_date":"2016-04-25","publish_status":"0","recid":"5537","relation_version_is_last":true,"title":["網膜虚血再灌流障害における分散ヘスペレチンの神経保護効果"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-09T06:58:04.126154+00:00"}