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Species identification and strain typing of dermatophytes by single-strand conformation polymorphism (SSCP) analysis of the ribosomal DNA and polymerase chain reaction analysis of subrepeat elements in the intergenic spacer region of Trichophyton rubrum
https://asahikawa-med.repo.nii.ac.jp/records/5178
https://asahikawa-med.repo.nii.ac.jp/records/51781905a937-9331-496a-9796-54347ede8766
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
6070.pdf (1.3 MB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||||||||||||||
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| 公開日 | 2015-04-28 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Species identification and strain typing of dermatophytes by single-strand conformation polymorphism (SSCP) analysis of the ribosomal DNA and polymerase chain reaction analysis of subrepeat elements in the intergenic spacer region of Trichophyton rubrum | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | jpn | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| キーワード | Dermatophytes | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| キーワード | Identification | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| キーワード | SSCP | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| キーワード | PCR | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| キーワード | Polymorphism | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 著者 |
Takahashi, Ichiro
× Takahashi, Ichiro
× Fukushima, Kazutaka
× Miyaji, Makoto
× Nishimura, Kazuko
× Asano, Kazuhiro
× Iizuka, Hajime
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| 書誌情報 |
旭川医科大学研究フォーラム 巻 15, 号 1, p. 27-36, 発行日 2015-02-01 |
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| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 1346-0102 | |||||||||||||||||
| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | The PCR-single strand conformation polymorphism (PCR-SSCP) analysis is a rapid and convenient technique for the detection of mutations and allelic variants. We applied this technique for the identification of various dermatophyte species. We also examined genetic polymorphisms in intra-species of 2 major dermatophytes, T. rubrum and T. mentgrophytes var. interdigitale. The PCR products including internal transcribed spacer (ITS) 1 region of ribosomal DNA (rDNA) of Trichophyton rubrum, T. mentagrophytes var. interdigitale, T. verrucosum, Microsporum canis, M. gypseum, Epidermophyton floccosum were analyzed. Specific SSCP patterns for each species were obtained. This result indicated that the system is useful for species identification. SSCP analysis of the amplified products from the posterior part of the D1/D2 (5′ end domain of the 26S rDNA) regions manifested the presence of 2 distinctive types of T. mentagrophytes var. interdigitale. The difference proved to be one nucleotide substitution, C → T. There was no clear correlation between the genotype patterns and morphological phenotypes of this fungus. On the other hand, no intra-species polymorphism was detected in T. rubrum by PCR-SSCP analysis using the region including ITS1, 5.8S rDNA, ITS2 and D1/D2, totally spanning about 1.2 kbp. Subsequent PCR analysis of non-transcribed intergenic spacer (IGS) region of T. rubrum showed the occurrence of numerous intra-species polymorphisms. The system should be useful for epidemiological surveillance of dermatophyte infection and give us new insights into pathogenicity of dermatophyte species. |
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| 注記 | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 注記 | 雑誌掲載版 | |||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||
| 資源タイプ | text | |||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||||
| ID(XooNIps) | ||||||||||||||||||
| f1501028 | ||||||||||||||||||
| 閲覧数(XooNIps) | ||||||||||||||||||
| ダウンロード数(XooNIps) | ||||||||||||||||||
| 1280 | ||||||||||||||||||
