{"created":"2023-06-19T11:24:27.635626+00:00","id":5178,"links":{},"metadata":{"_buckets":{"deposit":"c5eac2c6-a05d-40c0-84ed-79111132edfd"},"_deposit":{"created_by":1,"id":"5178","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"5178"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00005178","sets":["7","7:19","7:19:25"]},"author_link":["16189","16190","16185","16186","16188","16187"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-02-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"36","bibliographicPageStart":"27","bibliographicVolumeNumber":"15","bibliographic_titles":[{"bibliographic_title":"旭川医科大学研究フォーラム"}]}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The PCR-single strand conformation polymorphism (PCR-SSCP) analysis is a rapid and convenient technique for\nthe detection of mutations and allelic variants. We applied this technique for the identification of various dermatophyte\nspecies. We also examined genetic polymorphisms in intra-species of 2 major dermatophytes, T. rubrum and T.\nmentgrophytes var. interdigitale. The PCR products including internal transcribed spacer (ITS) 1 region of ribosomal DNA\n(rDNA) of Trichophyton rubrum, T. mentagrophytes var. interdigitale, T. verrucosum, Microsporum canis, M. gypseum,\nEpidermophyton floccosum were analyzed. Specific SSCP patterns for each species were obtained. This result indicated\nthat the system is useful for species identification. SSCP analysis of the amplified products from the posterior part of the\nD1/D2 (5′ end domain of the 26S rDNA) regions manifested the presence of 2 distinctive types of T. mentagrophytes var.\ninterdigitale. The difference proved to be one nucleotide substitution, C → T. There was no clear correlation between\nthe genotype patterns and morphological phenotypes of this fungus. On the other hand, no intra-species polymorphism\nwas detected in T. rubrum by PCR-SSCP analysis using the region including ITS1, 5.8S rDNA, ITS2 and D1/D2, totally\nspanning about 1.2 kbp. Subsequent PCR analysis of non-transcribed intergenic spacer (IGS) region of T. rubrum showed\nthe occurrence of numerous intra-species polymorphisms. The system should be useful for epidemiological surveillance of\ndermatophyte infection and give us new insights into pathogenicity of dermatophyte species.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"雑誌掲載版","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-0102","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"f1501028"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"1060"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"1280"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahashi, Ichiro"}],"nameIdentifiers":[{"nameIdentifier":"16185","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukushima, Kazutaka"}],"nameIdentifiers":[{"nameIdentifier":"16186","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyaji, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"16187","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishimura, Kazuko"}],"nameIdentifiers":[{"nameIdentifier":"16188","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Asano, Kazuhiro"}],"nameIdentifiers":[{"nameIdentifier":"16189","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iizuka, Hajime"}],"nameIdentifiers":[{"nameIdentifier":"16190","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"6070.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"6070.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/5178/files/6070.pdf"},"version_id":"22e7a893-16eb-4a2e-8d3e-1ab9d7659e84"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Dermatophytes","subitem_subject_scheme":"Other"},{"subitem_subject":"Identification","subitem_subject_scheme":"Other"},{"subitem_subject":"SSCP","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"Polymorphism","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Species identification and strain typing of dermatophytes by single-strand conformation polymorphism (SSCP) analysis of the ribosomal DNA and polymerase chain reaction analysis of subrepeat elements in the intergenic spacer region of Trichophyton rubrum","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Species identification and strain typing of dermatophytes by single-strand conformation polymorphism (SSCP) analysis of the ribosomal DNA and polymerase chain reaction analysis of subrepeat elements in the intergenic spacer region of Trichophyton rubrum"}]},"item_type_id":"5","owner":"1","path":["6","7","19","25"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-04-28"},"publish_date":"2015-04-28","publish_status":"0","recid":"5178","relation_version_is_last":true,"title":["Species identification and strain typing of dermatophytes by single-strand conformation polymorphism (SSCP) analysis of the ribosomal DNA and polymerase chain reaction analysis of subrepeat elements in the intergenic spacer region of Trichophyton rubrum"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-19T12:27:40.956208+00:00"}