| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2013-07-24 |
| タイトル |
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|
タイトル |
Critical roles of cold shock domain protein A as an endogenous angiogenesis inhibitor in skeletal muscle |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
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資源タイプ |
journal article |
| 著者 |
齊藤, 幸裕
Nakagami, Hironori
Azuma, Nobuyoshi
Hirata, Satoshi
Sanada, Fumihiro
Taniyama, Yoshiaki
Morishita, Ryuichi
Kaneda, Yasufumi
Sasajima, Tadahiro
|
| 書誌情報 |
Antioxidants & redox signaling
巻 15,
号 8,
p. 2109-2120,
発行日 2011-10-01
|
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
1523-0864 |
| DOI |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1089/ars.2010.3714 |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
21473684 |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Angiogenesis is regulated by the local balance between angiogenic stimulators and inhibitors and is maintained by muscle-derived angiogenic factors in ischemic tissues. AIMS: Our objectives were to investigate the effect of cold shock domain protein A (CSDA) as an endogenous angiogenesis inhibitor and to develop a novel strategy of therapeutic angiogenesis by blocking CSDA expression. RESULTS: In human skeletal muscle cells, CSDA was upregulated during hypoxia when cells were damaged and apoptosis was induced. CSDA expression could repress the activity of hypoxia inducible factor-1α and nuclear factor κB, because CSDA can competitively bind the hypoxia response element and the nuclear factor κB-binding element. As a result, vascular endothelial growth factor-A, interleukin-6, and interleukin-8 secretions from skeletal muscle cells were decreased. Further, CSDA depletion increased the secretion level of these angiogenic factors. In a hindlimb ischemia model, transfer of short-hairpin RNA targeting CSDA ameliorated ischemia without direct transfer of angiogenic factors. In this ischemic tissue, vascular endothelial growth factor-A, interleukin-6, and CXCL2 protein levels were increased. INNOVATION AND CONCLUSION: CSDA appears to play a critical role as an endogenous angiogenesis inhibitor in skeletal muscle, and RNA interference targeting of CSDA is a promising gene therapy strategy for treating peripheral arterial disease. |
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言語 |
en |
| 注記 |
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内容記述タイプ |
Other |
|
注記 |
Publisher |
| 資源タイプ |
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内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
VoR |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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21473684 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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|
563 |
5342.pdf (17.2 MB)
