| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2013-02-28 |
| タイトル |
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タイトル |
Production of offspring after sperm chromosome screening: an experiment using the mouse model |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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キーワード |
chromosome analysis |
| キーワード |
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主題Scheme |
Other |
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キーワード |
genetic diagnosis |
| キーワード |
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主題Scheme |
Other |
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キーワード |
sperm |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
渡部, 浩之
日下部, 博一
Mori, H
Yanagimachi, R
立野, 博幸
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| 著者 ローマ字 |
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Kusakabe, Hirokazu |
| 著者 ローマ字 |
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en |
| 著者 ローマ字 |
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Tateno, Hiroyuki |
| 著者 ローマ字 |
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en |
| 書誌情報 |
Human Reproduction
巻 28,
p. 531-537,
発行日 2013-01-01
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| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0268-1161 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1093/humrep/des388 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://humrep.oxfordjournals.org/content/28/2/531.long | http://humrep.oxfordjournals.org/content/28/2/531.long |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
23136143 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
STUDY QUESTION: Is it possible to produce offspring after sperm chromosome screening？ \nSUMMARY ANSWER: It is possible to produce zygotes after examining the genome of individual spermatozoa prior to embryo production. \nWHAT IS KNOWN ALREADY: Chromosomal aberrations in gametes are a major cause of pregnancy loss in women treated with assisted reproductive technology. However, to our knowledge, there are no reports on the successful genomic screening of spermatozoa, although some attempts have been made using the mouse as a model. \nSTUDY DESIGN: To prevent the transmission of chromosomal aberrations from fathers to offspring, we performed sperm chromosome screening (SCS) prior to fertilization using the mouse as a model. The production of offspring after SCS consists of (i) replication of the sperm chromosomes, (ii) analysis of one copy of the replicated sperm chromosomes, (iii) construction of a zygote using another set of chromosomes and (iv) production of a transferable embryo. \nMATERIALS, SETTING, METHODS: A single spermatozoon of a male mouse, with or without a Robertsonian translocation, was injected into an enucleated oocyte to allow the replication of sperm chromosomes. One of the sister blastomeres of a haploid androgenic 2-cell embryo was used for chromosome analysis. The other blastomere was fused with an unfertilized oocyte, activated and allowed to develop to a blastocyst before transfer to a surrogate mother. \nMAIN RESULTS AND ROLE OF CHANCE: With high efficiency, we were able to analyze sperm chromosomes in a blastomere from the androgenic 2-cell embryos and culture zygotes, with and without aberrant chromosomes, to the blastocyst stage before embryo transfer. The karyotypes of the offspring faithfully reflected those of the blastomeres used for SCS. \nLIMITATIONS, REASONS FOR CAUTION: This study was conducted using a mouse model; whether or not the method is applicable to humans is not known. \nWIDER IMPLICATIONS OF THE FINDINGS: This study has shown that it is possible to produce zygotes without any paternally inherited aberrations by examining the genome of individual spermatozoa prior to embryo production. |
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言語 |
en |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
Author |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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23136143 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1042 |
5072.pdf (6.2 MB)
