{"created":"2023-06-19T11:23:44.056481+00:00","id":4317,"links":{},"metadata":{"_buckets":{"deposit":"2e242808-efb9-45af-99f4-97f31d12be8c"},"_deposit":{"created_by":3,"id":"4317","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4317"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00004317","sets":["7","7:24"]},"author_link":["13455","13456","13457","13458","13459"],"control_number":"4317","item_1716186501932":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"23136143","subitem_relation_type_select":"PMID"}}]},"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"537","bibliographicPageStart":"531","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"Human Reproduction"}]}]},"item_5_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"http://humrep.oxfordjournals.org/content/28/2/531.long | http://humrep.oxfordjournals.org/content/28/2/531.long","subitem_description_type":"Other"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"STUDY QUESTION: Is it possible to produce offspring after sperm chromosome screening？ \\nSUMMARY ANSWER: It is possible to produce zygotes after examining the genome of individual spermatozoa prior to embryo production. \\nWHAT IS KNOWN ALREADY: Chromosomal aberrations in gametes are a major cause of pregnancy loss in women treated with assisted reproductive technology. However, to our knowledge, there are no reports on the successful genomic screening of spermatozoa, although some attempts have been made using the mouse as a model. \\nSTUDY DESIGN: To prevent the transmission of chromosomal aberrations from fathers to offspring, we performed sperm chromosome screening (SCS) prior to fertilization using the mouse as a model. The production of offspring after SCS consists of (i) replication of the sperm chromosomes, (ii) analysis of one copy of the replicated sperm chromosomes, (iii) construction of a zygote using another set of chromosomes and (iv) production of a transferable embryo. \\nMATERIALS, SETTING, METHODS: A single spermatozoon of a male mouse, with or without a Robertsonian translocation, was injected into an enucleated oocyte to allow the replication of sperm chromosomes. One of the sister blastomeres of a haploid androgenic 2-cell embryo was used for chromosome analysis. The other blastomere was fused with an unfertilized oocyte, activated and allowed to develop to a blastocyst before transfer to a surrogate mother. \\nMAIN RESULTS AND ROLE OF CHANCE: With high efficiency, we were able to analyze sperm chromosomes in a blastomere from the androgenic 2-cell embryos and culture zygotes, with and without aberrant chromosomes, to the blastocyst stage before embryo transfer. The karyotypes of the offspring faithfully reflected those of the blastomeres used for SCS. \\nLIMITATIONS, REASONS FOR CAUTION: This study was conducted using a mouse model; whether or not the method is applicable to humans is not known. \\nWIDER IMPLICATIONS OF THE FINDINGS: This study has shown that it is possible to produce zygotes without any paternally inherited aberrations by examining the genome of individual spermatozoa prior to embryo production.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"Author","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/humrep/des388","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0268-1161","subitem_source_identifier_type":"PISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"23136143"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"831"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"1042"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Kusakabe, Hirokazu"},{"subitem_text_language":"en"},{"subitem_text_value":"Tateno, Hiroyuki"},{"subitem_text_language":"en"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡部, 浩之","creatorNameLang":"ja"},{"creatorName":"ワタナベ, ヒロユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"13455","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"日下部, 博一","creatorNameLang":"ja"},{"creatorName":"クサカベ, ヒロカズ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"13456","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mori, H","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13457","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yanagimachi, R","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"13458","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"立野, 博幸","creatorNameLang":"ja"},{"creatorName":"タテノ, ヒロユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"13459","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"5072.pdf","filesize":[{"value":"6.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"5072.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/4317/files/5072.pdf"},"version_id":"23e3369c-3553-460d-b059-775286a737b1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"chromosome analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"genetic diagnosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sperm","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Production of offspring after sperm chromosome screening: an experiment using the mouse model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Production of offspring after sperm chromosome screening: an experiment using the mouse model","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["6","7","24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-02-28"},"publish_date":"2013-02-28","publish_status":"0","recid":"4317","relation_version_is_last":true,"title":["Production of offspring after sperm chromosome screening: an experiment using the mouse model"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-08-02T08:54:05.255510+00:00"}