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Ca^<2+> Release to Lumen from ADP-sensitive Phosphoenzyme E1PCa_2 without Bound K^+ of Sarcoplasmic Reticulum Ca^<2+>-ATPase
https://asahikawa-med.repo.nii.ac.jp/records/4042
https://asahikawa-med.repo.nii.ac.jp/records/40421200e256-8464-4798-9488-67874aa91bd1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
4496.pdf (4.4 MB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||||||||||||
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| 公開日 | 2012-05-17 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Ca^<2+> Release to Lumen from ADP-sensitive Phosphoenzyme E1PCa_2 without Bound K^+ of Sarcoplasmic Reticulum Ca^<2+>-ATPase | |||||||||||||||
| 言語 | en | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||
| 著者 |
山崎, 和生
× 山崎, 和生
× Daiho, Takashi
× Danko, Stefania
× Suzuki, Hiroshi
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| 書誌情報 |
Journal of Biological Chemistry 巻 285, 号 49, p. 38674-38683, 発行日 2010-12-01 |
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| ISSN | ||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||
| 収録物識別子 | 0021-9258 | |||||||||||||||
| DOI | ||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||
| 関連識別子 | 10.1074/jbc.M110.183343 | |||||||||||||||
| 抄録 | ||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||
| 内容記述 | During Ca^<2+> transport by sarcoplasmic reticulum Ca^<2+>-ATPase, the conformation change of ADP-sensitive phosphoenzyme (E1PCa_2) to ADP-insensitive phosphoenzyme (E2PCa_2) is followed by rapid Ca^<2+> release into the lumen. Here, we find that in the absence of K^+, Ca^<2+> release occurs considerably faster than E1PCa_2 to E2PCa_2 conformation change. Therefore, the lumenal Ca^<2+> release pathway is open to some extent in the K^+-free E1PCa_2 structure. The Ca^<2+> affinity of this E1P is as high as that of the unphosphorylated ATPase (E1), indicating the Ca^<2+> binding sites are not disrupted. Thus, bound K^+ stabilizes the E1PCa_2 structure with occluded Ca^<2+>, keeping the Ca^<2+> pathway to the lumen closed. We found previously (Yamasaki, K., Wang, G., Daiho, T., Danko, S., and Suzuki, H. (2008) J. Biol. Chem. 283, 29144-29155) that the K^+ bound in E2P reduces the Ca^<2+> affinity essential for achieving the high physiological Ca^<2+> gradient and to fully open the lumenal Ca^<2+> gate for rapid Ca^<2+> release (E2PCa_2 → E2P + 2Ca^<2+>). These findings show that bound K^+ is critical for stabilizing both E1PCa_2 and E2P structures, thereby contributing to the structural changes that efficiently couple phosphoenzyme processing and Ca^<2+> handling. | |||||||||||||||
| 言語 | en | |||||||||||||||
| 注記 | ||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||
| 注記 | © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||
| 資源タイプ | text | |||||||||||||||
| 著者版フラグ | ||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||
| ID(XooNIps) | ||||||||||||||||
| 20937807 | ||||||||||||||||
| 閲覧数(XooNIps) | ||||||||||||||||
| ダウンロード数(XooNIps) | ||||||||||||||||
| 828 | ||||||||||||||||
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Cite as
山崎, 和生, Daiho, Takashi, Danko, Stefania, Suzuki, Hiroshi, n.d., Ca^<2+> Release to Lumen from ADP-sensitive Phosphoenzyme E1PCa_2 without Bound K^+ of Sarcoplasmic Reticulum Ca^<2+>-ATPase: 38674–38683 p.
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