{"created":"2023-06-19T11:23:29.563042+00:00","id":4042,"links":{},"metadata":{"_buckets":{"deposit":"f10dfd11-a14b-44ce-8c0c-28728456e19e"},"_deposit":{"created_by":3,"id":"4042","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4042"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00004042","sets":["7","7:24"]},"author_link":["12297","12298","12299","12300"],"control_number":"4042","item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"49","bibliographicPageEnd":"38683","bibliographicPageStart":"38674","bibliographicVolumeNumber":"285","bibliographic_titles":[{"bibliographic_title":"Journal of Biological Chemistry"}]}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"During Ca^<2+> transport by sarcoplasmic reticulum Ca^<2+>-ATPase, the conformation change of ADP-sensitive phosphoenzyme (E1PCa_2) to ADP-insensitive phosphoenzyme (E2PCa_2) is followed by rapid Ca^<2+> release into the lumen. Here, we find that in the absence of K^+, Ca^<2+> release occurs considerably faster than E1PCa_2 to E2PCa_2 conformation change. Therefore, the lumenal Ca^<2+> release pathway is open to some extent in the K^+-free E1PCa_2 structure. The Ca^<2+> affinity of this E1P is as high as that of the unphosphorylated ATPase (E1), indicating the Ca^<2+> binding sites are not disrupted. Thus, bound K^+ stabilizes the E1PCa_2 structure with occluded Ca^<2+>, keeping the Ca^<2+> pathway to the lumen closed. We found previously (Yamasaki, K., Wang, G., Daiho, T., Danko, S., and Suzuki, H. (2008) J. Biol. Chem. 283, 29144-29155) that the K^+ bound in E2P reduces the Ca^<2+> affinity essential for achieving the high physiological Ca^<2+> gradient and to fully open the lumenal Ca^<2+> gate for rapid Ca^<2+> release (E2PCa_2 → E2P + 2Ca^<2+>). These findings show that bound K^+ is critical for stabilizing both E1PCa_2 and E2P structures, thereby contributing to the structural changes that efficiently couple phosphoenzyme processing and Ca^<2+> handling.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1074/jbc.M110.183343","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9258","subitem_source_identifier_type":"PISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"20937807"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"1135"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"828"}]},"item_5_version_type_38":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山崎, 和生","creatorNameLang":"ja"},{"creatorName":"ヤマザキ, カズオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"12297","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Daiho, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"12298","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Danko, Stefania","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"12299","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"12300","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"4496.pdf","filesize":[{"value":"4.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"4496.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/4042/files/4496.pdf"},"version_id":"18c13bf1-ef4b-46eb-9f59-e3513fd83b90"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ca^<2+> Release to Lumen from ADP-sensitive Phosphoenzyme E1PCa_2 without Bound K^+ of Sarcoplasmic Reticulum Ca^<2+>-ATPase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ca^<2+> Release to Lumen from ADP-sensitive Phosphoenzyme E1PCa_2 without Bound K^+ of Sarcoplasmic Reticulum Ca^<2+>-ATPase","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["6","7","24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-05-17"},"publish_date":"2012-05-17","publish_status":"0","recid":"4042","relation_version_is_last":true,"title":["Ca^<2+> Release to Lumen from ADP-sensitive Phosphoenzyme E1PCa_2 without Bound K^+ of Sarcoplasmic Reticulum Ca^<2+>-ATPase"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-07-30T01:24:38.514080+00:00"}