| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2012-05-17 |
| タイトル |
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タイトル |
Preparation of highlypurifiedmonomeric human serum albumin as secondary reference material for standardization of urinary albumin immunoassays |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
伊藤, 喜久
Ichihara, Kiyoshi
Kish, Kouji
Hosogaya, Shigem
Yamada, Toshiyuki
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| 書誌情報 |
Clinica Chimica Act
巻 413,
号 1-2,
p. 175-181,
発行日 2012-01-01
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| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0009-8981 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.cca.2011.09.016 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
【図表は本人の意向により載せません 】 | 【図表は本人の意向により載せません 】 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Background : International external quality assessments have shown variation in results of urinary albumin among various immunoassays. A well-defined candidate reference material for urine albumin (cRM-UA) was prepared to improve standardization. Methods : cRM-UA was prepared from a commercially available preparation of human serum albumin by using gel-filtration HPLC. The value was assigned by transfer from ERM-DA470 using immunoassay systems qualified based on the linearity and variability observed in dilution tests of pooled urine and the calibrators. Effectiveness of recalibration using the cRM-UA was evaluated by measuring 129 urine specimens. Results : The cRM-UA had a monomeric albumin peak which accounted for 98.9% of the total area by gel filtration HPLC. The lyophilized preparation of the cRM-UA had suitable homogeneity, and short- and long-term stability. Nine of 14 immunoassays met the criteria were used for value assignment. The assigned concentration was 225.1 ± 9.11 mg/l [mean ± U: expanded uncertainty with k = 2] when reconstituted with 3.00 ml of purified water on weight basis. Recalibration of 7 qualified immunoassays using the cRM-UA resulted in between-method CV of 6.6%. Conclusions : The cRM-UA was successful in achieving standardization of urine albumin results among 7 immunoassays which possess performance attributes representing uniform reactivity to both cRM-UA and clinical urine samples. |
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言語 |
en |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
http://dx.doi.org/10.1016/j.cca.2011.09.016 |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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21963465 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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791 |
4464.pdf (378.6 kB)
