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Preparation of highlypurifiedmonomeric human serum albumin as secondary reference material for standardization of urinary albumin immunoassays
https://asahikawa-med.repo.nii.ac.jp/records/4005
https://asahikawa-med.repo.nii.ac.jp/records/400582d9def8-7889-462c-92da-06909e49a4a5
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4464.pdf (378.6 kB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||||||||||||||
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| 公開日 | 2012-05-17 | |||||||||||||||||
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| タイトル | Preparation of highlypurifiedmonomeric human serum albumin as secondary reference material for standardization of urinary albumin immunoassays | |||||||||||||||||
| 言語 | en | |||||||||||||||||
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| 言語 | eng | |||||||||||||||||
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| 資源タイプ | journal article | |||||||||||||||||
| 著者 |
伊藤, 喜久
× 伊藤, 喜久
× Ichihara, Kiyoshi
× Kish, Kouji
× Hosogaya, Shigem
× Yamada, Toshiyuki
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| 書誌情報 |
Clinica Chimica Act 巻 413, 号 1-2, p. 175-181, 発行日 2012-01-01 |
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| 収録物識別子タイプ | PISSN | |||||||||||||||||
| 収録物識別子 | 0009-8981 | |||||||||||||||||
| DOI | ||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | 10.1016/j.cca.2011.09.016 | |||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | 【図表は本人の意向により載せません 】 | 【図表は本人の意向により載せません 】 | |||||||||||||||||
| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Background : International external quality assessments have shown variation in results of urinary albumin among various immunoassays. A well-defined candidate reference material for urine albumin (cRM-UA) was prepared to improve standardization. Methods : cRM-UA was prepared from a commercially available preparation of human serum albumin by using gel-filtration HPLC. The value was assigned by transfer from ERM-DA470 using immunoassay systems qualified based on the linearity and variability observed in dilution tests of pooled urine and the calibrators. Effectiveness of recalibration using the cRM-UA was evaluated by measuring 129 urine specimens. Results : The cRM-UA had a monomeric albumin peak which accounted for 98.9% of the total area by gel filtration HPLC. The lyophilized preparation of the cRM-UA had suitable homogeneity, and short- and long-term stability. Nine of 14 immunoassays met the criteria were used for value assignment. The assigned concentration was 225.1 ± 9.11 mg/l [mean ± U: expanded uncertainty with k = 2] when reconstituted with 3.00 ml of purified water on weight basis. Recalibration of 7 qualified immunoassays using the cRM-UA resulted in between-method CV of 6.6%. Conclusions : The cRM-UA was successful in achieving standardization of urine albumin results among 7 immunoassays which possess performance attributes representing uniform reactivity to both cRM-UA and clinical urine samples. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 注記 | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 注記 | http://dx.doi.org/10.1016/j.cca.2011.09.016 | |||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||
| 資源タイプ | text | |||||||||||||||||
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| 出版タイプ | AM | |||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||||
| ID(XooNIps) | ||||||||||||||||||
| 21963465 | ||||||||||||||||||
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| 791 | ||||||||||||||||||
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伊藤, 喜久, Ichihara, Kiyoshi, Kish, Kouji, Hosogaya, Shigem, Yamada, Toshiyuki, n.d., Preparation of highlypurifiedmonomeric human serum albumin as secondary reference material for standardization of urinary albumin immunoassays: 175–181 p.
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