| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2007-02-27 |
| タイトル |
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タイトル |
TNF-α induced RANTES chemokine expression via activation of NF-kB and p38 MAP kinase: roles of TNF-α in alcoholic liver diseases |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
平野, 史倫
Komura, K
Fukawa, E
Makino, I
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| 著者 ローマ字 |
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Hirano, Fuminori |
| 書誌情報 |
JOURNAL OF HEPATOLOGY
巻 38,
号 4,
p. 483-489,
発行日 2003-04-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0168-8278 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/S0168-8278(02)00456-7 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://www.sciencedirect.com/science/journal/01688278 | http://www.sciencedirect.com/science/journal/01688278 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Background/Aims: Increased concentration of plasma TNF-α correlates with the clinical course of alcoholic liver diseases. In addition, hepatic RANTES which migrates CD4 T lymphocytes to liver is increased in patients with alcoholic hepatitis. We investigated that roles of TNF -α on RANTES expression in hepatocytes. Methods: HLE cells were treated with TNF-α in the presence, or absence of several inhibitors. Enzyme-linked immunoassay and reverse transcriptase-polymerase chain reaction were performed for the measurement of protein production and mRNA of RANTES, respectively. Moreover, DNA-binding activity of NF-kB was investigated using electrophoretic mobility shift assay. To exam effects of TNF-α on RANTES gene expression, luciferase assay was performed. Results: TNF-α clearly up-regulated RANTES expression in a time-dependent fashion and induced DNA-binding activity of NF-kB. Moreover, TNF-α -induced RANTES expression was completely inhibited by SB203580, but not calphostin C and wortmannin. Luciferase assay showed that TNF-α increased RANTES gene expression and mutation of NF-kB binding sites in the RANTES promoter ablated TNF-α inducibility. Conclusions: We presented that RANTES was transcriptionally induced in human hepatoma cells by treatment with TNF-α via activation of NF-kB and p38 MAP kinase, presumably suggesting that TNF-α-induced expression of RANTES plays important roles in cell-mediated liver injury in alcoholic liver diseases. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
ELSEVIER, Hirano, F; Komura, K; Fukawa, E; Makino, I, JOURNAL OF HEPATOLOGY, 38(4), 483-489, 2003.
\nauthor |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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12663241 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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866 |
256.pdf (464.1 kB)
