| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2009-04-24 |
| タイトル |
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タイトル |
Thyroid-stimulating hormone induces interleukin-18 gene expression in FRTL-5 cells: immunohistochemical detection of interleukin-18 in autoimmune thyroid disease. |
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言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
滝山, 由美
Miyokawa, N
Tokusashi, Y
Ito, K
Kato, S
Kimura, S
Sato, K
Katagiri, M
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| 著者 ローマ字 |
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Takiyama, Yumi |
| 著者 ローマ字 |
|
|
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en |
| 書誌情報 |
Thyroid
巻 12,
号 11,
p. 935-943,
発行日 2002-11-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1050-7256 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1089/105072502320908268 |
| リンクURL |
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内容記述タイプ |
Other |
|
内容記述 |
http://www.liebertonline.com/loi/thy | http://www.liebertonline.com/loi/thy |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Interleukin(IL)‐l8 is a cloned cytokine that was identified originally as a factor having potent interferon(IFN)‐γ-inducing activity on Kupffer cells. First, we analyzed lL-18 gene expression by reverse transcription-polymerase chain reaction(RT-PCR) in rat thyroid FRTL-5 cells and human thyroid tissue samples. The expression of IL-18mRNA in FRTL-5 cells was enhanced by thryoid-stimulating hormone(TSH) in a dose-dependent manner. 8-Bromo-cyclic adenosine monophosphate(cAMP） also increased in lL-18 mRNA levels. Furthermore, TGCT clones that exhibited an increase in intracellular cAMP accumulation showed an increased IL-l8 mRNA signal when compared to controls. Taken together, these data suggested that the effect of TSH on IL-18 gene expression was mediated by activating protein kinase A. Treatment of FRTL-5 cells with the antithyroid drug, methimazole(MMI), suppressed this stimulatory action of TSH on IL-18 gene expression. Next, we examined IL-18 expression in human thyroid tissue derived from patients with autoimmune thyroid diseases(ATD). RTPCR and immunohistology demonstrated that human thyroid follicular cells expressed lL-18. Especially in thyroid tissue from a patient with Hashimoto's thyroiditis, expression was more diffuse and extensive, generally observed in close relation to a lymphocytic infiltrate. Also, IL-18 protein was distributed in the same follicles that express Fas-L and HLA-DR. This study is the first to demonstrate the detection of lL-l8 in the thyroid gland. The frequent expression of IL-l8 in thyrocytes suggests that IL-18 itself might be a secreted immunomodulator in ATD． |
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言語 |
en |
| 注記 |
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内容記述タイプ |
Other |
|
注記 |
Copyright 2002 Mary Ann Liebert, Inc. publisher |
|
言語 |
en |
| 資源タイプ |
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内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
VoR |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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|
12490070 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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|
796 |
2026.pdf (1.9 MB)
