| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2009-03-31 |
| タイトル |
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タイトル |
Inhibitors of the Ca^<2+>/calmodulin-dependent protein kinase phosphatase family (CaMKP and CaMKP-N) |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
石田, 敦彦
Sueyoshi, N
Takao, T
Nimura, T
Sugiyama, Y
Numano, T
Shigeri, Y
Taniguchi, T
Kameshita, I
|
| 著者 ローマ字 |
|
|
|
Ishida, Atsuhiko |
| 書誌情報 |
Biochemical and Biophysical Research Communications
巻 363,
号 3,
p. 715-721,
発行日 2007-11-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0006-291X |
| DOI |
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関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.bbrc.2007.09.022 |
| リンクURL |
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内容記述タイプ |
Other |
|
内容記述 |
http://dx.doi.org/10.1016/j.bbrc.2007.09.022 | http://dx.doi.org/10.1016/j.bbrc.2007.09.022 |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Ca^<2+>/calmodulin-dependent protein kinase phosphatase (CaMKP) and its nuclear isoform CaMKP-N are unique Ser/Thr protein phosphatases that negatively regulate the Ca^<2+>/calmodulin-dependent protein kinase (CaMK) cascade by dephosphorylating multifunctional CaMKI, II, and IV. However, the lack of specific inhibitors of these phosphatases has hampered studies on these enzymes in vivo. In an attempt to obtain specific inhibitors, we searched inhibitory compounds and found that Evans Blue and Chicago Sky Blue 6B served as effective inhibitors for CaMKP. These compounds also inhibited CaMKP-N, but inhibited neither protein phosphatase 2C, another member of PPM family phosphatase, nor calcineurin, a typical PPP family phosphatase. The minimum structure required for the inhibition was 1-amino-8-naphthol-4-sulfonic acid. When Neuro2a cells cotransfected with CaMKIV and CaMKP-N were treated with these compounds, the dephosphorylation of CaMKIV was strongly suppressed, suggesting that these compounds could be used as potent inhibitors of CaMKP and CaMKP-N in vivo as well as in vitro. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
author |
| 資源タイプ |
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内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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17897624 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1784 |
1903.pdf (352.3 kB)
