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Mouse and human spermatozoa can be freeze-dried without damaging their chromosomes
https://asahikawa-med.repo.nii.ac.jp/records/1338
https://asahikawa-med.repo.nii.ac.jp/records/133896079410-d94c-4932-af33-3afcd9f145e8
| 名前 / ファイル | ライセンス | アクション |
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1623.pdf (391.6 kB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||||||||||
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| 公開日 | 2009-03-11 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Mouse and human spermatozoa can be freeze-dried without damaging their chromosomes | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 著者 |
日下部, 博一
× 日下部, 博一
× Yanagimachi, R
× Kamiguchi, Y
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| 著者 ローマ字 | ||||||||||||||
| Kusakabe, Hirokazu | ||||||||||||||
| 書誌情報 |
Human Reproduction 巻 23, 号 2, p. 233-239, 発行日 2008-02-01 |
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| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0268-1161 | |||||||||||||
| DOI | ||||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | 10.1093/humrep/dem252 | |||||||||||||
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| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | http://dx.doi.org/10.1093/humrep/dem252 | http://dx.doi.org/10.1093/humrep/dem252 | |||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | BACKGROUND: Although mouse spermatozoa can be freeze-dried without losing their reproductive capacity, the technique needs further improvements to reduce the incidence of chromosomal damages to spermatozoa. Effects of freeze-drying on human spermatozoa are unknown. METHODS: Mouse spermatozoa were suspended in a Tris-buffered EGTA solution briefly (about 10 min at 37℃) or for 1-7 days at 4℃ before freeze-drying. Freeze-dried spermatozoa were maintained for up to 1 year at 4℃ before injection. Sperm chromosomes were examined during the first mitosis (cleavage) of zygotes. Sperm’s ability to support embryo development was assessed by examining mid-gestation fetuses after transfer of 2-cell embryos to surrogate mothers. Chromosome integrity of freeze-dried human spermatozoa was examined by injecting individual spermatozoa into mouse oocytes which were previously enucleated. RESULTS: When mouse spermatozoa were freeze-dried immediately after suspension in Tris-buffered EGTA solution, only about 40% had normal chromosomes. When the spermatozoa were kept in the same solution for 3-7 days before freeze-drying, 85-95% had normal chromosomes and they were able to support embryo development better than those which were in the solution briefly. Freeze-dried human spermatozoa well maintained their chromosomes regardless of the duration of pre-freeze-drying incubation of spermatozoa in the Tris-buffered EGTA solution. CONCLUSIONS: Prior incubation of mouse spermatozoa in Tris-buffered EGTA solution for several days makes sperm chromosomes more resistant to freeze-drying. As the consequence, spermatozoa freeze-dried this way support embryo development better than those exposed to Tris-buffered EGTA solution only briefly. Freeze-dried human spermatozoa well maintained their chromosomes without pre-freeze-drying incubation in Tris-buffered EGTA solution. | |||||||||||||
| 注記 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 注記 | This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Kusakabe, H. ; Yanagimachi, R. ; Kamiguchi, Y., Mouse and human spermatozoa can be freeze-dried without damaging their chromosomes, Human Reproduction 23(2), FEB 2008, pp. 233-239, is available online at: http://humrep.oxfordjournals.org/cgi/content/abstract/23/2/233 \nauthor |
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| 内容記述タイプ | Other | |||||||||||||
| 資源タイプ | text | |||||||||||||
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| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | application/pdf | |||||||||||||
| ID(XooNIps) | ||||||||||||||
| 18056060 | ||||||||||||||
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| 1385 | ||||||||||||||
