{"created":"2023-06-19T11:21:11.331878+00:00","id":1338,"links":{},"metadata":{"_buckets":{"deposit":"ebb3b376-c944-4480-857d-294c92a493fa"},"_deposit":{"created_by":3,"id":"1338","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1338"},"status":"published"},"_oai":{"id":"oai:asahikawa-med.repo.nii.ac.jp:00001338","sets":["6:29:30","7","7:24"]},"author_link":["4316","4317","4318"],"item_5_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-02-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"239","bibliographicPageStart":"233","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"Human Reproduction"}]}]},"item_5_description_25":{"attribute_name":"リンクURL","attribute_value_mlt":[{"subitem_description":"http://dx.doi.org/10.1093/humrep/dem252 | http://dx.doi.org/10.1093/humrep/dem252","subitem_description_type":"Other"}]},"item_5_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND: Although mouse spermatozoa can be freeze-dried without losing their reproductive capacity, the technique needs further improvements to reduce the incidence of chromosomal damages to spermatozoa. Effects of freeze-drying on human spermatozoa are unknown. METHODS: Mouse spermatozoa were suspended in a Tris-buffered EGTA solution briefly (about 10 min at 37℃) or for 1-7 days at 4℃ before freeze-drying. Freeze-dried spermatozoa were maintained for up to 1 year at 4℃ before injection. Sperm chromosomes were examined during the first mitosis (cleavage) of zygotes. Sperm’s ability to support embryo development was assessed by examining mid-gestation fetuses after transfer of 2-cell embryos to surrogate mothers. Chromosome integrity of freeze-dried human spermatozoa was examined by injecting individual spermatozoa into mouse oocytes which were previously enucleated. RESULTS: When mouse spermatozoa were freeze-dried immediately after suspension in Tris-buffered EGTA solution, only about 40% had normal chromosomes. When the spermatozoa were kept in the same solution for 3-7 days before freeze-drying, 85-95% had normal chromosomes and they were able to support embryo development better than those which were in the solution briefly. Freeze-dried human spermatozoa well maintained their chromosomes regardless of the duration of pre-freeze-drying incubation of spermatozoa in the Tris-buffered EGTA solution. CONCLUSIONS: Prior incubation of mouse spermatozoa in Tris-buffered EGTA solution for several days makes sperm chromosomes more resistant to freeze-drying. As the consequence, spermatozoa freeze-dried this way support embryo development better than those exposed to Tris-buffered EGTA solution only briefly. Freeze-dried human spermatozoa well maintained their chromosomes without pre-freeze-drying incubation in Tris-buffered EGTA solution.","subitem_description_type":"Abstract"}]},"item_5_description_36":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Kusakabe, H. ; Yanagimachi, R. ; Kamiguchi, Y., Mouse and human spermatozoa can be freeze-dried without damaging their chromosomes, Human Reproduction 23(2), FEB 2008, pp. 233-239, is available online at: http://humrep.oxfordjournals.org/cgi/content/abstract/23/2/233\n\\nauthor","subitem_description_type":"Other"}]},"item_5_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_5_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/humrep/dem252","subitem_relation_type_select":"DOI"}}]},"item_5_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0268-1161","subitem_source_identifier_type":"ISSN"}]},"item_5_text_44":{"attribute_name":"ID(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"18056060"}]},"item_5_text_54":{"attribute_name":"閲覧数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"2445"}]},"item_5_text_55":{"attribute_name":"ダウンロード数(XooNIps)","attribute_value_mlt":[{"subitem_text_value":"1385"}]},"item_5_text_7":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Kusakabe, Hirokazu"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"日下部, 博一"},{"creatorName":"クサカベ, ヒロカズ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"4316","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yanagimachi, R"}],"nameIdentifiers":[{"nameIdentifier":"4317","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamiguchi, Y"}],"nameIdentifiers":[{"nameIdentifier":"4318","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-07-06"}],"displaytype":"detail","filename":"1623.pdf","filesize":[{"value":"391.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1623.pdf","url":"https://asahikawa-med.repo.nii.ac.jp/record/1338/files/1623.pdf"},"version_id":"f6092e30-fc4b-4c04-866e-998fbe8a12cb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Mouse and human spermatozoa can be freeze-dried without damaging their chromosomes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mouse and human spermatozoa can be freeze-dried without damaging their chromosomes","subitem_title_language":"en"}]},"item_type_id":"5","owner":"3","path":["7","24","30"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-03-11"},"publish_date":"2009-03-11","publish_status":"0","recid":"1338","relation_version_is_last":true,"title":["Mouse and human spermatozoa can be freeze-dried without damaging their chromosomes"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-04-19T11:30:05.836240+00:00"}