| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2008-04-28 |
| タイトル |
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タイトル |
Fertilizability and chromosomal integrity of frozen-thawed Bryde's whale (Balaenoptera edeni) spermatozoa intracytoplasmically injected into mouse oocytes |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
立野, 裕幸
Watanabe, H
Tateno, H
Kusakabe, H
Matsuoka, T
Kamiguchi, Y
Fujise, Y
Ishikawa, H
Ohsumi, S
Fukui, Y
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| 著者 ローマ字 |
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|
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Tateno, Hiroyuki |
| 書誌情報 |
Zygote
巻 15,
号 1,
p. 9-14,
発行日 2007-02-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0967-1994 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1017/S0967199406003923 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://journals.cambridge.org/action/displayJournal?jid=ZYG | http://journals.cambridge.org/action/displayJournal?jid=ZYG |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Prior to attempting the in vitro production of embryos in the Bryde’s whale (Balaenoputera edeni), we investigated whether spermatozoa can retain the capacity for oocyte activation and pronucleus formation as well as chromosomal integrity under cryopreservation by using intracytoplasmic sperm injection (ICSI) into mouse oocytes. Regardless of motility and viability, whale spermatozoa efficiently led to the activation of mouse oocytes (90.3–97.4%), and sperm nuclei successfully transformed into male pronucleuswithin activated ooplasm (87.2–93.6%). Chromosome analysis at the first cleavage metaphase (M) of the hybrid zygotes revealed that a majority (95.2%) of motile spermatozoa had the normal chromosome complement, while the percentage of chromosomal normality was significantly reduced to 63.5% in immotile spermatozoa and 50.0% in dead spermatozoa due to the increase in structural chromosome aberrations. This is the first report showing that motile Bryde’s whale spermatozoa are competent to support embryonic development. |
| 注記 |
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内容記述タイプ |
Other |
|
注記 |
Copyright © 2007 Cambridge University Press |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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17391541 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1830 |
981.pdf (176.3 kB)
