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Fertilizability and chromosomal integrity of frozen-thawed Bryde's whale (Balaenoptera edeni) spermatozoa intracytoplasmically injected into mouse oocytes

https://asahikawa-med.repo.nii.ac.jp/records/794
https://asahikawa-med.repo.nii.ac.jp/records/794
417635ad-3903-4b67-9e32-74ef8ec77684
名前 / ファイル ライセンス アクション
981.pdf 981.pdf (176.3 kB)
Item type 学術雑誌論文 / Journal Article_02(1)
公開日 2008-04-28
タイトル
タイトル Fertilizability and chromosomal integrity of frozen-thawed Bryde's whale (Balaenoptera edeni) spermatozoa intracytoplasmically injected into mouse oocytes
言語 en
言語
言語 eng
資源タイプ
資源タイプ journal article
著者 立野, 裕幸

× 立野, 裕幸

立野, 裕幸

ja-Kana タテノ, ヒロユキ

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Watanabe, H

× Watanabe, H

Watanabe, H

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Tateno, H

× Tateno, H

Tateno, H

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Kusakabe, H

× Kusakabe, H

Kusakabe, H

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Matsuoka, T

× Matsuoka, T

Matsuoka, T

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Kamiguchi, Y

× Kamiguchi, Y

Kamiguchi, Y

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Fujise, Y

× Fujise, Y

Fujise, Y

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Ishikawa, H

× Ishikawa, H

Ishikawa, H

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Ohsumi, S

× Ohsumi, S

Ohsumi, S

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Fukui, Y

× Fukui, Y

Fukui, Y

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著者 ローマ字
Tateno, Hiroyuki
書誌情報 Zygote

巻 15, 号 1, p. 9-14, 発行日 2007-02-01
ISSN
収録物識別子タイプ ISSN
収録物識別子 0967-1994
DOI
識別子タイプ DOI
関連識別子 10.1017/S0967199406003923
リンクURL
内容記述タイプ Other
内容記述 http://journals.cambridge.org/action/displayJournal?jid=ZYG | http://journals.cambridge.org/action/displayJournal?jid=ZYG
抄録
内容記述タイプ Abstract
内容記述 Prior to attempting the in vitro production of embryos in the Bryde’s whale (Balaenoputera edeni), we investigated whether spermatozoa can retain the capacity for oocyte activation and pronucleus formation as well as chromosomal integrity under cryopreservation by using intracytoplasmic sperm injection (ICSI) into mouse oocytes. Regardless of motility and viability, whale spermatozoa efficiently led to the activation of mouse oocytes (90.3–97.4%), and sperm nuclei successfully transformed into male pronucleuswithin activated ooplasm (87.2–93.6%). Chromosome analysis at the first cleavage metaphase (M) of the hybrid zygotes revealed that a majority (95.2%) of motile spermatozoa had the normal chromosome complement, while the percentage of chromosomal normality was significantly reduced to 63.5% in immotile spermatozoa and 50.0% in dead spermatozoa due to the increase in structural chromosome aberrations. This is the first report showing that motile Bryde’s whale spermatozoa are competent to support embryonic development.
注記
内容記述タイプ Other
注記 Copyright © 2007 Cambridge University Press
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内容記述タイプ Other
資源タイプ text
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17391541
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Cite as

立野, 裕幸, Watanabe, H, Tateno, H, Kusakabe, H, Matsuoka, T, Kamiguchi, Y, Fujise, Y, Ishikawa, H, Ohsumi, S, Fukui, Y, n.d., Fertilizability and chromosomal integrity of frozen-thawed Bryde's whale (Balaenoptera edeni) spermatozoa intracytoplasmically injected into mouse oocytes: 9–14 p.

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