| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2008-03-28 |
| タイトル |
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|
タイトル |
DNA differential diagnosis of taeniasis and cysticercosis by multiplex PCR |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
伊藤, 亮
Yamasaki, H
Allan, JC
Sato, MO
Sako, Y
Nakaya, K
Dongchuan, Q
Nakao, M
Mamuti, W
Craig, PS
|
| 著者 ローマ字 |
|
|
|
Ito, Akira |
| 書誌情報 |
Journal of Clinical Microbiology
巻 42,
号 2,
p. 548-553,
発行日 2004-02-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0095-1137 |
| DOI |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1128/JCM.42.2.548-553.2004 |
| リンクURL |
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内容記述タイプ |
Other |
|
内容記述 |
http://www.ncbi.nlm.nih.gov/pubmed/14766815 | http://www.ncbi.nlm.nih.gov/pubmed/14766815 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Multiplex PCR was established for differential diagnosis of taeniasis and cysticercosis, including their causative agents. For identification of the parasites, multiplex PCR with cytochrome c oxidase subunit 1 gene yielded evident differential products unique for Taenia saginata and Taenia asiatica and for American/African and Asian genotypes of Taenia solium with molecular sizes of 827, 269, 720, and 984 bp, respectively. In the PCR-based detection of tapeworm carriers using fecal samples, the diagnostic markers were detected from 7 of 14 and 4 of 9 T. solium carriers from Guatemala and Indonesia, respectively. Test sensitivity may have been reduced by the length of time (up to 12 years) that samples were stored and/or small sample volumes (ca. 30 to 50 mg). However, the diagnostic markers were detected by nested PCR in five worm carriers from Guatemalan cases that were found to be negative by multiplex PCR. It was noteworthy that a 720 bp-diagnostic marker was detected from a T. solium carrier who was egg-free, implying that it is possible to detect worm carriers and treat before mature gravid proglottids are discharged. In contrast to T. solium carriers, 827-bp markers were detected by multiplex PCR in all T. saginata carriers. The application of the multiplex PCR would be useful not only for surveillance of taeniasis and cysticercosis control but also for the molecular epidemiological survey of these cestode infections. |
| 注記 |
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|
内容記述タイプ |
Other |
|
注記 |
Copyright © American Society for Microbiology, Journal of Clinical Microbiology, volume 42, 548-553, 2004
\n著者版 |
| 資源タイプ |
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|
内容記述タイプ |
Other |
|
資源タイプ |
text |
| フォーマット |
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|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| ID(XooNIps) |
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14766815 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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|
1608 |
913.pdf (486.2 kB)
