| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2008-03-28 |
| タイトル |
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タイトル |
Neuronal calcium sensor protein visinin-like protein-3 interacts with microsomal cytochrome b5 in a Ca2+-dependent manner. |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
及川, 賢輔
Kimura, S
Aoki, N
Atsuta, Y
Takiyama, Y
Nagato, T
Yanai, M
Kobayashi, H
Sato, K
Sasajima, T
Tateno, H
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| 著者 ローマ字 |
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Oikawa, Kensuke |
| 書誌情報 |
Journal of Biological Chemistry
巻 279,
号 15,
p. 15142-15152,
発行日 2004-04-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0021-9258 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1074/jbc.M312766200 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://www.ncbi.nlm.nih.gov/pubmed?term=Neuronal%20calcium%20sensor%20protein%20visinin-like%20protein-3%20interacts%20with%20microsomal%20cytochrome%20b5%20in%20a%20Ca2%2B-dependent%20manner. | http://www.ncbi.nlm.nih.gov/pubmed?term=Neuronal%20calcium%20sensor%20protein%20visinin-like%20protein-3%20interacts%20with%20microsomal%20cytochrome%20b5%20in%20a%20Ca2%2B-dependent%20manner. |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Visinin-like protein-3, which is one of the neuronal calcium sensors, has been shown to be mainly expressed in cerebellar Purkinje cells, but cellular function of this protein has not yet been elucidated. We examined the tissue distribution of murine visinin-like protein-3 transcripts using real-time reverse transcription-PCR. Visinin-like protein-3 mRNA was found to be expressed in peripheral tissues. Particularly, the expression of the transcript in the thymus was significantly higher than in other peripheral tissues. In addition, B6RVTC1 thymoma cells robustly expressed visinin-like protein-3 mRNA. To identify a target protein of visinin-like protein-3, we performed a pull-down experiment using glutathione S-transferase-tagged visinin-like protein-3 and two-dimensional electrophoresis. We demonstrated that microsomal cytochrome b(5) was a Ca(2+)-dependent binding partner of visinin-like protein-3. In a co-immunoprecipitation experiment, it was observed that hippocalcin, as well as visinin-like protein-3, could interact with cytochrome b(5). Furthermore, we confirmed that the sequence Val(114)-Tyr(127) at the C-terminal tail of cytochrome b(5) is the minimal structural requirement for binding to visinin-like protein-3. In addition, the loop His(19)-His(25) at the N terminus of visinin-like protein-3 is essential for binding to cytochrome b(5). Microsomal cytochrome b(5) was also shown to be a potential activator of cytochrome P450. The present findings raise the possibility that visinin-like protein-3 may link Ca(2+) signaling to the machinery of microsomal monooxygenase complex composed of cytochrome b(5), cytochrome P450, and some reductases. This report provides the first evidence of an interaction between visinin-like protein-3 and microsomal cytochrome b(5). |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
Copyright 2004 by The American Society for Biochemistry and Molecular Biology, Inc.
\nauthor |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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14739275 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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832 |
893.pdf (451.4 kB)
