| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2008-02-28 |
| タイトル |
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タイトル |
Characterization of Entry Mechanisms of Human Herpesvirus 8 by Using an Rta-Dependent Reporter Cell Line. |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
古谷野, 伸
Inoue, N
Winter, J
Lal, RB
Offermann, MK
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| 著者 ローマ字 |
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Koyano, Shin |
| 書誌情報 |
Journal of Virology
巻 77,
号 14,
p. 8147-8152,
発行日 2003-01-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0022-538X |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1128/JVI.77.14.8147-8152.2003 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
To analyze the mechanisms of entry of human herpesvirus 8 (HHV-8), we established a reporter cell line T1H6 that contains the lacZ gene under the control of the polyadenylated nuclear RNA promoter, known to be strongly activated by a viral transactivator, Rta. We found that infection with cell-free virus, as well as cocultivation with HHV-8-positive primary effusion lymphoma cell lines, activated the lacZ gene of T1H6 in a sensitive and dose-dependent manner. Addition of Polybrene and centrifugation enhanced, but polysulfonate compounds inhibited, the HHV-8 infectivity. RGD-motif-containing polypeptides and integrins did not decrease the infectivity, suggesting the presence of an additional cellular receptor other than the reported one. The entry was dependent on pH acidification but not on the clathrin pathway. Although conditioned media obtained from human immunodeficiency virus (HIV)-infected cells did not have any effect on the early steps of HHV-8 infection, intracellular expression of a proviral HIV type 1, but not of Tat alone, increased the HHV-8-dependent reporter activation slightly, suggesting a potential of HIV-mediated enhancement of an early step of HHV-8 infection. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
Copyright © American Society for Microbiology, Journal of Virology, volume 77, 8147-8152, 2003
\npublisher |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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12829853 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1590 |
869.pdf (205.0 kB)
