| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2008-02-28 |
| タイトル |
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タイトル |
Evaluation of an enzyme-linked immunosorbent assay (ELISA) with affinity-purified Em18 and an ELISA with recombinant Em18 for differential diagnosis of alveolar echinococcosis: results of a blind test. |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
伊藤, 亮
Xiao, N
Liance, M
Sato, MO
Sako, Y
Mamuti, W
Ishikawa, Y
Nakao, M
Yamasaki, H
Nakaya, K
Bardonnet, K
Bresson, HS
Vuitton, DA
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| 著者 ローマ字 |
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Ito, Akira |
| 書誌情報 |
Journal of Clinical Microbiology
巻 40,
号 11,
p. 4161-4165,
発行日 2002-11-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0095-1137 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1128/JCM.40.11.4161-4165.2002 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://www.ncbi.nlm.nih.gov/pubmed?term=Evaluation%20of%20an%20enzyme-linked%20immunosorbent%20assay%20%28ELISA%29%20with%20affinity-purified%20Em18%20and%20an%20ELISA%20with%20recombinant%20Em18%20for%20differential%20diagnosis%20of%20alveolar%20echinococcosis%3A%20results%20of%20a%20blind%20test. | http://www.ncbi.nlm.nih.gov/pubmed?term=Evaluation%20of%20an%20enzyme-linked%20immunosorbent%20assay%20%28ELISA%29%20with%20affinity-purified%20Em18%20and%20an%20ELISA%20with%20recombinant%20Em18%20for%20differential%20diagnosis%20of%20alveolar%20echinococcosis%3A%20results%20of%20a%20blind%20test. |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Alveolar echinococcosis (AE) is the most potentially lethal parasitic zoonosis of the nontropical areas in the northern hemisphere, where cystic echinococcosis (CE) is also endemic. Both AE and CE are highly endemic in China, and both serologic detection of echinococcosis, either AE or CE, and differentiation of AE from CE are crucial problems. Evaluation of Western blot analysis (WB) and enzyme-linked immunosorbent assay (ELISA) for the Em18 antigen, using affinity-purified and recombinant Em18, was carried out "blindly" using 60 human sera from patients diagnosed in France. The results were compared with those obtained using a commercially available Echinococcus WB immunoglobulin G (IgG) kit developed in France. The Em18 WB and Echinococcus WB IgG showed very similar results for detection of AE. Both affinity-purified Em18 or a recombinant Em18 WB and Echinococcus WB IgG seem useful for identification of AE, and the latter seems appropriate for both AE and CE, whereas affinity-purified Em18 ELISA and the newly developed recombinant Em18 ELISA appear to be suitable for detection of AE, especially for epidemiological surveys. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
Copyright © American Society for Microbiology, Journal of Clinical Microbiology, volume 40, 4161-4165, 2002
\npublisher |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
VoR |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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12409391 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1064 |
862.pdf (232.7 kB)
