| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2007-12-03 |
| タイトル |
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タイトル |
A cytogenetic study of in-vitro matured murine oocytes after ICSI by human sperm |
|
言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
千石, 一雄
Tsuchiya, K
Kamiguchi, Y
Ishikawa, M
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| 著者 ローマ字 |
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Sengoku, Kazuo |
| 書誌情報 |
Human Reproduction
巻 17,
号 2,
p. 420-425,
発行日 2002-02-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0268-1161 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1093/humrep/17.2.420 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://humrep.oxfordjournals.org/cgi/reprint/17/2/420 | http://humrep.oxfordjournals.org/cgi/reprint/17/2/420 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
BACKGROUND: The purpose of this study was to investigate the chromosomal complement and developmental potential of in-vitro matured murine oocytes following ICSI by human sperm. METHODS: Heterologous ICSI fertilization between mouse oocytes and human sperm was employed in order to overcome the reduced fertilization rates observed after conventional IVF due to zona hardening during in-vitro maturation, and to assess separately maternal and paternal chromosome complements. Cytogenetic analyses were performed in four types of oocytes: (I) in-vitro matured metaphase II (MII) oocytes; (ii) in-vivo matured MII oocytes; (iii) in-vitro matured oocytes after ICSI; (iv) in-vivo matured oocytes after ICSI. RESULTS: Activation rates after ICSI of in-vitro matured oocytes was lower than that of in-vivo matured oocytes (69.9 versus 97.2%, P < 0.01), and premature chromosomal condensation was only observed in in-vitro matured oocytes. However, there were no significant differences in developmental rates after successful activation between in-vivo and in-vitro matured ICSI oocytes (69.7 versus 76.6%). The incidences of aneuploidy and structural aberrations were similar between the ICSI embryos and non-ICSI (MII) oocytes. Furthermore, the frequency of chromosomal aberrations was not associated with in-vitro or in-vivo maturation. Similar analyses of paternal chromosomes indicated that there were no significant differences in the incidence of chromosomal aberrations between the embryos derived from in-vitro and in-vivo matured oocytes. CONCLUSIONS: These results suggest that in-vitro matured oocytes following ICSI do not lead to an increase in the frequency of aneuploidy and structural aberrations when human sperm are injected into mouse oocytes. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Oxford University Press, Human Reproduction, 17(2), 2002, 420-425 is available online at: http://humrep.oxfordjournals.org/cgi/content/abstract/17/2/420.
\nauthor |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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11821288 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1003 |
800.pdf (183.6 kB)
