| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2007-12-03 |
| タイトル |
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タイトル |
Epidermal lamellar granules transport different cargoes as distinct aggregates |
|
言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
山本, 明美
Simon, M
Kishibe, M
Miyauchi, Y
Takahashi, H
Yoshida, S
O'Brien, TJ
Serre, G
Iizuka, H
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| 著者 ローマ字 |
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Yamamoto(Ishida), Akemi |
| 書誌情報 |
Journal of Investigative Dermatology
巻 122,
号 5,
p. 1137-1144,
発行日 2004-05-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0022-202X |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1111/j.0022-202X.2004.22515.x |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://www.nature.com/jid/journal/v122/n5/full/5602318a.html | http://www.nature.com/jid/journal/v122/n5/full/5602318a.html |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Lamellar granules (LG) of the epidermis appear as discrete round or oblong shaped granules in classical transmission electron micrographs, but a recent cryo-transmission electron microscopy study has claimed that LG are in fact branched tubular structures. LG contain various cargoes including lipids, hydrolytic enzymes, and several other proteins. It is not known whether there are any differences in the timing of expression among them and whether they are sorted into the granules individually or collectively. In order to address these questions, we studied the expression of glucosylceramides (GlcCer), cathepsin D (CatD), corneodesmosin (Cdsn), kallikrein (KLK)7, and KLK8 in normal human epidermis using confocal laser scanning microscopy and immunoelectron microscopy. The results were consistent with the model that LG are parts of a branched tubular structure. In this structure, all the components were shown to be distributed as separate aggregates. In the trans-Golgi network (TGN), bulbous protrusions containing GlcCer, Cdsn, KLK7 and KLK8, and small CatD-positive vesicles were observed. The molecules were shown to be delivered to the apical region of granular keratinocytes. This study provides strong evidence for the sequential synthesis and independent trafficking of various LG cargoes, including for the first time CatD and KLK8, from TGN. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
Nature Publishing Group, Journal of Investigative Dermatology, 122, 5, 2004, 1137-1144
\nauthor |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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15140216 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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913 |
789.pdf (2.1 MB)
