| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2018-06-08 |
| タイトル |
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タイトル |
Incomplete KLK7 Secretion and Upregulated LEKTI Expression Underlie Hyperkeratotic Stratum Corneum in Atopic Dermatitis |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
井川, 哲子
岸部, 麻里
堀, 仁子
本間, 大
辻村, 久
石川, 准子
村上, 正基
山本, 明美
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| 著者 ローマ字 |
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Igawa, Satoko |
| 著者 ローマ字 |
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Kishibe, Mari |
| 著者 ローマ字 |
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Hori-Minami, Masako |
| 著者 ローマ字 |
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Honma, Masaru |
| 著者 ローマ字 |
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Tsujimura, Hisashi |
| 著者 ローマ字 |
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Ishikawa, Junko |
| 著者 ローマ字 |
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Murakami, Masamoto |
| 著者 ローマ字 |
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Ishida-Yamamoto, Akemi |
| 書誌情報 |
Journal of Investigative Dermatology
巻 137,
号 2,
p. 449-456,
発行日 2017-02-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0022-202X |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.jid.2016.10.015 |
| 識別番号 その他 |
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内容記述タイプ |
Other |
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内容記述 |
PMID：27769847 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Atopic dermatitis (AD) is a common inflammatory skin disorder. Chronic AD lesions present hyperkeratosis, indicating a disturbed desquamation process. KLK7 is a serine protease involved in the proteolysis of extracellular corneodesmosome components, including desmocollin 1 and corneodesmosin, which leads to desquamation. KLK7 is secreted by lamellar granules and upregulated in AD lesional skin. However, despite increased KLK7 protein levels, immunostaining and electron microscopy indicated numerous corneodesmosomes remaining in the uppermost layer of the stratum corneum from AD lesions. We aimed to clarify the discrepancy between KLK7 overexpression and retention of corneodesmosomes on AD corneocytes. Western blot analysis indicated abnormal corneodesmosin degradation patterns in stratum corneum from AD lesions. The KLK activity of tape-stripped corneocytes from AD lesions was not significantly elevated in in situ zymography, which was our new attempt to detect the protease activity more precisely than conventional assays. This ineffective KLK activation was associated with impaired KLK7 secretion from lamellar granules and increased expression of LEKTI in AD. Such imbalances in protease-protease inhibitor interactions could lead to abnormal proteolysis of corneodesmosomes and compact hyperkeratosis. Upregulated expression of LEKTI might be a compensatory mechanism to prevent further barrier dysfunction in AD. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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27769847 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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308 |
7010.pdf (10.6 MB)
