| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2016-09-13 |
| タイトル |
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|
タイトル |
The Behavior and Acrosomal Status of Mouse Spermatozoa In Vitro, and Within the Oviduct During Fertilization after Natural Mating |
| 言語 |
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|
言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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キーワード |
acrosome reaction |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
cumulus oophorus |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
fertilization |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
hyperactivated motility |
| キーワード |
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|
主題Scheme |
Other |
|
キーワード |
mouse |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
oviduct |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
sperm |
| キーワード |
|
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主題Scheme |
Other |
|
キーワード |
zona pellucida |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
日野, 敏昭
室, 悠子
中野（田村）, 美和
岡部, 勝
立野, 裕幸
柳町, 隆造
|
| 著者 ローマ字 |
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|
|
Hino, Toshiaki |
| 著者 ローマ字 |
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Muro, Yuko |
| 著者 ローマ字 |
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Tamura-Nakano, Miwa |
| 著者 ローマ字 |
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Okabe, Masaru |
| 著者 ローマ字 |
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Tateno, Hiroyuki |
| 著者 ローマ字 |
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Yanagimachi, Ryuzo |
| 書誌情報 |
Biology of Reproduction
巻 95,
号 3,
p. 50,
発行日 2016-09-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0006-3363 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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|
関連識別子 |
10.1095/biolreprod.116.140400 |
| 識別番号 その他 |
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内容記述タイプ |
Other |
|
内容記述 |
PMID:27417908 |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Although 90%-100% of mouse oocytes can be fertilized in vitro with capacitated spermatozoa within 1 h after insemination, oocytes within the oviduct are fertilized one by one over a period of several hours. In vitro experiments showed that both acrosome-intact and acrosome-reacted spermatozoa entered the cumulus oophorus, but that acrosome-reacted spermatozoa reached the surface of oocytes more readily than acrosome-intact spermatozoa. During the period of fertilization within the oviduct, acrosome-reacted spermatozoa were seen throughout the isthmus, but with higher incidence in the upper than in the mid- and lower segments of the isthmus. Very few spermatozoa were present in the ampulla, and almost all were acrosome reacted. Although the cumulus oophorus and zona pellucida are known to be able to induce or facilitate the acrosome reaction of spermatozoa, this picture makes it likely that almost all fertilizing mouse spermatozoa within the oviduct begin to react before ascending from the isthmus to the ampulla. We witnessed a reacted spermatozoon that stayed on the zona pellucida of a fertilized oocyte for a while; it then moved out of the cumulus before reaching the zona pellucida of the nearby unfertilized oocyte. We noted that only a few spermatozoa migrate from the isthmus to the ampulla during the progression of fertilization, and this must be one of the reasons why we do not see many spermatozoa swarming around a single oocyte during in vivo fertilization. |
| 資源タイプ |
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|
内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
VoR |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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27417908 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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|
649 |
6539.pdf (4.1 MB)
