| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2007-08-24 |
| タイトル |
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タイトル |
Stimulation of Ca^<2+>/calmodulin-dependent protein kinase phosphatase by polycations |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
石田, 敦彦
Kameshita, I
Kitani, T
Okuno, S
Takeuchi, M
Fujisawa, H
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| 著者 ローマ字 |
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Ishida, Atsuhiko |
| 書誌情報 |
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
巻 408,
号 2,
p. 229-238,
発行日 2002-12-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0003-9861 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/S0003-9861(02)00592-1 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://www.science-direct.com/science/journal/00039861 | http://www.science-direct.com/science/journal/00039861 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Ca^<2+>/calmodulin-dependent protein kinase phosphatase (CaMKPase) dephosphorylates and regulates multifunctional Ca^<2+>/calmodulin-dependent protein kinases (CaMKs). One of the prominent features of CaMKPase is stimulation of phosphatase activity by polycations such as poly--lysine (poly(Lys)). Using various polycations, basicity and molecular weight of the polymer proved to be important for the stimulation. Surface plasmon resonance (SPR) analysis showed that CaMKIV(T196D), which mimics CaMKPase substrate, and CaMKPase could form tight complexes with poly(Lys). Pull-down binding experiments suggested that the formation of a tightly associated ternary complex consisting of CaMKPase, poly(Lys), and phosphorylated CaMKIV is essential for stimulation. Dilution experiments also supported this contention. Poly(Lys) failed to stimulate a CaMKPase mutant in which a Glu cluster corresponding to residues 101–109 in the N-terminal domain was deleted, and the mutant could not interact with poly(Lys) in the presence of Mn^<2+>. Thus, the Glu cluster appeared to be the binding site for polycations and to play a pivotal role in the polycation stimulation of CaMKPase activity. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
Elsevier, Ishida, A. ; Kameshita, I. ; Kitani, T. ; Okuno, S. ; Takeuchi, M. ; Fujisawa, H., ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 408(2), 2002, 229-238 |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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12464276 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1406 |
715.pdf (262.6 kB)
