| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2007-08-24 |
| タイトル |
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タイトル |
Generation of a polyclonal antibody that simultaneously detects multiple Ser/Thr protein kinases |
|
言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
石田, 敦彦
Kameshita, I
Kinoshita, S
Shigeri, Y
Tatsu, Y
Yumoto, N
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| 著者 ローマ字 |
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Ishida, Atsuhiko |
| 書誌情報 |
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
巻 60,
号 1,
p. 13-22,
発行日 2004-07-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0165-022X |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.jbbm.2004.03.006 |
| リンクURL |
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内容記述タイプ |
Other |
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内容記述 |
http://www.science-direct.com/science/journal/0165022X | http://www.science-direct.com/science/journal/0165022X |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
In order to obtain a polyclonal antibody that recognizes various protein kinases, a peptide corresponding to an amino acid sequence of a highly conserved subdomain (subdomain VIB) of the protein kinase family was synthesized and used for immunization. When the synthetic peptide, CVVHRDLKPENLLLAS, was coupled to keyhole limpet hemocyanin (KLH) and used to immunize rabbits, polyclonal antibodies that detected multiple protein kinases on a Western blot were generated. One of the antibodies obtained, KI98, detected a variety of purified Ser/Thr protein kinases, such as calmodulin-dependent protein kinase II (CaM-kinase II), calmodulin-dependent protein kinase IV (CaM-kinase IV), cAMP-dependent protein kinase, protein kinase C, and Erk2. The antibody detected as low as 0.2 ng of protein kinases blotted onto a nitrocellulose membrane by dot-immunobinding assay. When a rat brain extract was analyzed with this antibody, various protein kinases were simultaneously detected. The present anti-peptide antibody with a broad spectrum of cross-reactivity to multiple protein kinases may be a powerful tool for comprehensive analysis focused on protein kinases. |
| 注記 |
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内容記述タイプ |
Other |
|
注記 |
Elsevier, Isamu Kameshita, Shun Kinoshita, Yasushi Shigeri, Yoshiro Tatsu, Noboru Yumoto, Atsuhiko Ishida, JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 60(1), 2004, 13-22
\nauthor |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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15236906 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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1005 |
712.pdf (463.0 kB)
