| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2017-09-12 |
| タイトル |
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タイトル |
Comparison of the Diagnostic Accuracy of Three Rapid Tests for the Serodiagnosis of Hepatic Cystic Echinococcosis in Humans. |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
Francesca, Tamarozzi
Ilaria, Covini
Mara, Mariconti
Roberta, Narra
Carmine, Tinelli
Annalisa, De Silvestri
Federica, Manzoni
Adriano, Casulli
伊藤, 亮
Andreas, Neumayr
Enrico, Brunetti
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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Ito, Akira |
| 書誌情報 |
PLoS neglected tropical diseases
巻 10,
号 2,
p. e0004444,
発行日 2016-02-01
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| 識別番号 その他 |
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内容記述タイプ |
Other |
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内容記述 |
PMID：26871432 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
BACKGROUND:
\nThe diagnosis of cystic echinococcosis (CE) is based primarily on imaging, in particular with ultrasound for abdominal CE, complemented by serology when imaging results are unclear. In rural endemic areas, where expertise in ultrasound may be scant and conventional serology techniques are unavailable due to lack of laboratory equipment, Rapid Diagnostic Tests (RDTs) are appealing.
METHODOLOGY/PRINCIPAL FINDINGS:
\nWe evaluated the diagnostic accuracy of 3 commercial RDTs for the diagnosis of hepatic CE. Sera from 59 patients with single hepatic CE cysts in well-defined ultrasound stages (gold standard) and 25 patients with non-parasitic cysts were analyzed by RDTs VIRapid HYDATIDOSIS (Vircell, Spain), Echinococcus DIGFA (Unibiotest, China), ADAMU-CE (ICST, Japan), and by RIDASCREEN Echinococcus IgG ELISA (R-Biopharm, Germany). Sensitivity, specificity and ROC curves were compared with McNemar and t-test. For VIRapid and DIGFA, correlation between semiquantitative results and ELISA OD values were evaluated by Spearman's coefficient. Reproducibility was assessed on 16 randomly selected sera with Cohen's Kappa coefficient. Sensitivity and Specificity of VIRapid (74%, 96%) and ADAMU-CE (57%, 100%) did not differ from ELISA (69%, 96%) while DIGFA (72%, 72%) did (p = 0.045). ADAMU-CE was significantly less sensitive in the diagnosis of active cysts (p = 0.019) while DIGFA was significantly less specific (p = 0.014) compared to ELISA. All tests were poorly sensitive in diagnosing inactive cysts (33.3% ELISA and ADAMU-CE, 42.8% DIGFA, 47.6% VIRapid). The reproducibility of all RDTs was good-very good. Band intensity of VIRapid and DIGFA correlated with ELISA OD values (r = 0.76 and r = 0.79 respectively, p<0.001).
CONCLUSIONS/SIGNIFICANCE:
\nRDTs may be useful in resource-poor settings to complement ultrasound diagnosis of CE in uncertain cases. VIRapid test appears to perform best among the examined kits, but all tests are poorly sensitive in the presence of inactive cysts, which may pose problems with accurate diagnosis. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
This is an open access article distributed under the terms of the Creative Commons Attribution License |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
VoR |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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26874132 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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872 |
6782.pdf (2.1 MB)
