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Simple Identification of Human Taenia Species by Multiplex Loop-Mediated Isothermal Amplification in Combination with Dot Enzyme-Linked Immunosorbent Assay
https://asahikawa-med.repo.nii.ac.jp/records/5695
https://asahikawa-med.repo.nii.ac.jp/records/569585520461-0b48-492e-933b-728f0346b93a
| 名前 / ファイル | ライセンス | アクション |
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6531.pdf (2.9 MB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||||||||||||||||
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| 公開日 | 2016-08-19 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | Simple Identification of Human Taenia Species by Multiplex Loop-Mediated Isothermal Amplification in Combination with Dot Enzyme-Linked Immunosorbent Assay | |||||||||||||||||||
| 言語 | ||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| 著者 |
Agathe, Nkouawa
× Agathe, Nkouawa
× 迫, 康仁
× 岡本, 宗裕
× 伊藤, 亮
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| 著者 ローマ字 | ||||||||||||||||||||
| 著者 ローマ字 | ||||||||||||||||||||
| Sako, Yasuhito | ||||||||||||||||||||
| 著者 ローマ字 | ||||||||||||||||||||
| Okamoto, Munehiro | ||||||||||||||||||||
| 著者 ローマ字 | ||||||||||||||||||||
| Ito, Akira | ||||||||||||||||||||
| 書誌情報 |
The American journal of tropical medicine and hygiene 巻 94, 号 6, p. 1318-1323, 発行日 2016-06-01 |
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| ISSN | ||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 0002-9637 | |||||||||||||||||||
| DOI | ||||||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||
| 関連識別子 | 10.4269/ajtmh.15-0829 | |||||||||||||||||||
| 識別番号 その他 | ||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||
| 内容記述 | PMID:27044566 | |||||||||||||||||||
| 抄録 | ||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||
| 内容記述 | For differential detection of Taenia solium, Taenia saginata, and Taenia asiatica, loop-mediated isothermal amplification (LAMP) assay targeting the cytochrome c oxidase subunit 1 gene has been recently developed and shown to be sensitive, specific, and effective. However, to achieve differential identification, one specimen requires three reaction mixtures containing a primer set of each Taenia species separately, which is complex and time consuming and increases the risk of cross-contamination. In this study, we developed a simple differential identification of human Taenia species using multiplex LAMP (mLAMP) in combination with dot enzyme-linked immunosorbent assay (dot-ELISA). Forward inner primers of T. solium, T. saginata, and T. asiatica labeled with fluorescein isothiocyanate (FITC), digoxigenin (DIG), and tetramethylrhodamine (TAMRA), respectively, and biotin-labeled backward inner primers were used in mLAMP. The mLAMP assay succeeded in specific amplification of each respective target gene in a single tube. Furthermore, the mLAMP product from each species was easily distinguished by dot-ELISA with an antibody specific for FITC, DIG, or TAMRA. The mLAMP assay in combination with dot-ELISA will make identification of human Taenia species simpler, easier, and more practical. | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||
| 資源タイプ | text | |||||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||||||
| ID(XooNIps) | ||||||||||||||||||||
| 27044566 | ||||||||||||||||||||
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| 383 | ||||||||||||||||||||
