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Trpm7 Protein Contributes to Intercellular Junction Formation in Mouse Urothelium
https://asahikawa-med.repo.nii.ac.jp/records/5685
https://asahikawa-med.repo.nii.ac.jp/records/5685a4cd457c-a18c-42f6-9978-bcfe153bd17b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
6743.pdf (3.0 MB)
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| Item type | 学位論文 / Thesis or Dissertation_02(1) | |||||||||
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| 公開日 | 2017-06-30 | |||||||||
| タイトル | ||||||||||
| タイトル | Trpm7 Protein Contributes to Intercellular Junction Formation in Mouse Urothelium | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | animal model | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | cell junction | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | epithelial cell | |||||||||
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| 主題Scheme | Other | |||||||||
| 主題 | inflammation | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | transient receptor potential channels (TRP channels) | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| その他（別言語等）のタイトル | ||||||||||
| その他のタイトル | TRPM7蛋白のマウス尿路上皮細胞間結合の形成への関与 | |||||||||
| 著者 |
渡邊, 成樹
× 渡邊, 成樹
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| 著者 ローマ字 | ||||||||||
| Watanabe, Masaki | ||||||||||
| 書誌情報 |
The Journal of biological chemistry. 巻 290, 号 50, p. 29882-29892, 発行日 2015-12-01 |
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| DOI | ||||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1074/jbc.M115.667899 | |||||||||
| 識別番号 その他 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | PMID：26504086 | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲512 | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2017-06-30 | |||||||||
| 学位名 | ||||||||||
| 学位名 | 博士（医学） | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関名 | 旭川医科大学 | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Trpm7 is a divalent cation-permeable channel that has been reported to be involved in magnesium homeostasis as well as cellular adhesion and migration. We generated urothelium-specific Trpm7 knock-out (KO) mice to reveal the function of Trpm7 in vivo. A Trpm7 KO was induced by tamoxifen and was confirmed by genomic PCR and immunohistochemistry. By using patch clamp recordings in primary urothelial cells, we observed that Mg(2+)-inhibitable cation currents as well as acid-inducible currents were significantly smaller in Trpm7 KO urothelial cells than in cells from control mice. Assessment of voiding behavior indicated a significantly smaller voided volume in Trpm7 KO mice (mean voided volume 0.28 ± 0.08 g in KO mice and 0.36 ± 0.04 g in control mice, p < 0.05, n = 6-8). Histological analysis showed partial but substantial edema in the submucosal layer of Trpm7 KO mice, most likely due to inflammation. The expression of proinflammatory cytokines TNF-α and IL-1β was significantly higher in Trpm7 KO bladders than in controls. In transmission electron microscopic analysis, immature intercellular junctions were observed in Trpm7 KO urothelium but not in control mice. These results suggest that Trpm7 is involved in the formation of intercellular junctions in mouse urothelium. Immature intercellular junctions in Trpm7 knock-out mice might lead to a disruption of barrier function resulting in inflammation and hypersensitive bladder afferent nerves that may affect voiding behavior in vivo. | |||||||||
| 資源タイプ | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| フォーマット | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
