| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2016-05-02 |
| タイトル |
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タイトル |
The three isoforms of hepcidin in human serum and their processing determined by liquid chromatography-tandem mass spectrometry (LC-tandem MS) |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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キーワード |
Hepcidin |
| キーワード |
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主題Scheme |
Other |
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キーワード |
Hepcidin isoforms |
| キーワード |
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主題Scheme |
Other |
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キーワード |
LC |
| キーワード |
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主題Scheme |
Other |
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キーワード |
Tandem MS |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
Addo, Lynda
生田, 克哉
田中, 宏樹
土岐, 康通
畑山, 真弓
山本, 昌代
伊藤, 巧
進藤, 基博
佐々木, 祐介
下中, 靖
藤谷, 幹浩
高後, 裕
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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Ikuta, Katsuya |
| 著者 ローマ字 |
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Tanaka, Hiroki |
| 著者 ローマ字 |
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Toki, Yasumichi |
| 著者 ローマ字 |
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Hatayama, Mayumi |
| 著者 ローマ字 |
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Yamamoto, Masayo |
| 著者 ローマ字 |
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Ito, Satoshi |
| 著者 ローマ字 |
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Shindo, Motohiro |
| 著者 ローマ字 |
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Sasaki, Yusuke |
| 著者 ローマ字 |
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Shimonaka, Yasushi |
| 著者 ローマ字 |
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Fujiya, Mikihiro |
| 著者 ローマ字 |
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Kohgo, Yutaka |
| 書誌情報 |
International Journal of Hematology
巻 103,
号 1,
p. 34-43,
発行日 2016-01-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0925-5710 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1007/s12185-015-1885-y |
| 識別番号 その他 |
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内容記述タイプ |
Other |
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内容記述 |
PMID:26462810 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Hepcidin, the iron regulatory hormone, has three isoforms; -20, -22 and -25. While hepcidin-25 has been studied extensively, the physiological significance of other isoforms remains poorly understood. Using a quantitative method based on liquid chromatography-tandem mass spectrometry (LC-tandem MS) developed by our group, we quantified hepcidin isoforms in human serum to elucidate their characteristics, and investigated the role of hepatocytes in isoform processing. Hepcidin isoforms in serum obtained from 40 healthy volunteers were quantified. Synthetic hepcidin peptides were added to healthy serum, and to HepG2 culture media, and hepcidin isoform concentrations determined. All three hepcidin isoforms were detected in human serum; however, hepcidin-25 concentrations were highest. The three hepcidin isoforms showed a strong positive correlation with each other and with serum ferritin. Additionally, while hepcidin-20 was strongly correlated with serum creatinine, the other isoforms were not. Hepcidin-20 and -25 levels were also increased in chronic kidney disease (CKD) serum. Hepcidin-22 rapidly degraded into hepcidin-20, whereas hepcidin-25 remained relatively stable. Finally, hepcidin-22 degradation into hepcidin-20 was accelerated in the presence of HepG2. This method has enabled us to reveal fundamental characteristics of the three hepcidin isoforms in serum and may be a powerful tool for quantifying hepcidin isoform expression and processing. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
The final publication is available at Springer via http://dx.doi.org/10.1007/s12185-015-1885-y |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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26462810 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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728 |
6416.pdf (650.0 kB)
