| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2016-03-16 |
| タイトル |
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|
タイトル |
Downregulation of miR-15a due to LMP1 promotes cell proliferation and predicts poor prognosis in nasal NK/T-cell lymphoma. |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Nasal natural killer/T cell lymphoma |
| キーワード |
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|
主題Scheme |
Other |
|
キーワード |
microRNA-15a |
| キーワード |
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|
主題Scheme |
Other |
|
キーワード |
Epstein-Barr virus |
| キーワード |
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|
主題Scheme |
Other |
|
キーワード |
v-myb myeloblastosis viral oncogene homolog |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
cyclin D1 |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Epstein-Barr virus latent membrane protein 1 |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
駒林, 優樹
岸部, 幹
長門, 利純
上田, 征吾
高原, 幹
原渕, 保明
|
| 著者 ローマ字 |
|
|
|
Komabayashi, Yuki |
| 著者 ローマ字 |
|
|
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Kishibe, Kan |
| 著者 ローマ字 |
|
|
|
Nagato, Toshihiro |
| 著者 ローマ字 |
|
|
|
Ueda, Seigo |
| 著者 ローマ字 |
|
|
|
Takahara, Miki |
| 著者 ローマ字 |
|
|
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Harabuchi, Yasuaki |
| 書誌情報 |
American journal of hematology
巻 89,
号 1,
p. 25-33,
発行日 2014-01-01
|
| ISSN |
|
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0361-8609 |
| DOI |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1002/ajh.23570. |
| リンクURL |
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内容記述タイプ |
Other |
|
内容記述 |
http://onlinelibrary.wiley.com/doi/10.1002/ajh.23570/full | http://onlinelibrary.wiley.com/doi/10.1002/ajh.23570/full |
| 識別番号 その他 |
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|
内容記述タイプ |
Other |
|
内容記述 |
PMID:23963825 |
| 抄録 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
Nasal NK/T-cell lymphoma (NNKTL) is an Epstein-Barr virus (EBV)-associated malignancy and has distinct clinical and histological features. However, its genetic features are hitherto unclear. MicroRNAs (miRNAs) play a crucial role in the pathogenesis of several malignancies via regulating gene expression. In this study, we investigated whether the specific microRNAs were related to the tumor behaviors in NNKTL. MiRNA array and Quantitative RT-PCR analyses revealed that miR-15a was expressed at a much lower level in NNKTL cells (SNK-1, SNK-6, and SNT-8) than in normal peripheral NK cells and EBV-negative NK cell line KHYG-1. Quantitative PCR and western blot analyses showed that the expression of MYB and cyclin D1, which are validated targets of miR-15a, was higher in NNKTL cells. Transfection of NNKTL cells (SNK-6 and SNT-8) with a miR-15a precursor decreased MYB and cyclin D1 levels, thereby blocking G1/S transition and cell proliferation. Knockdown of EBV-encoded latent membrane protein 1 (LMP1) significantly increased miR-15a expression in SNK-6 cells. In NNKTL tissues, we found that reduced miR-15a expression, which correlated with MYB and cyclin D1 expression, was associated with poor prognosis of NNKTL patients. These data suggest that downregulation of miR-15a, possibly due to LMP1, implicates in the pathogenesis of NNKTL by inducing cell proliferation via MYB and cyclin D1. Thus, miR-15a could be a potential target for antitumor therapy and a prognostic predictor for NNKTL. |
| 資源タイプ |
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内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| ID(XooNIps) |
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23963825 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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|
1172 |
6365.pdf (1.7 MB)
