| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2015-05-18 |
| タイトル |
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タイトル |
Contributions of hepatocytes and bile ductular cells in ductular reactions and remodeling of the biliary system after chronic liver injury. |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
永濱, 康晴
Sone, Masayuki
Xi, Chen
岡田, 陽子
山本, 雅大
Bing, Xin
Matsuo, Yasuhiro
Komatsu, Mikiko
Suzuki, Akira
Enomoto, Katsuhiko
西川, 祐司
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| 著者 ローマ字 |
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Nagahama, Yasuharu |
| 著者 ローマ字 |
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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Okada, Yoko |
| 著者 ローマ字 |
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Yamamoto, Masahiro |
| 著者 ローマ字 |
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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| 著者 ローマ字 |
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Nishikawa, Yuji |
| 書誌情報 |
The American Journal of Pathology
巻 184,
号 11,
p. 3001-3012,
発行日 2014-11-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0002-9440 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.ajpath.2014.07.005 |
| 識別番号 その他 |
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内容記述タイプ |
Other |
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内容記述 |
PMID:25193593 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Mature hepatocytes are suggested to possess a capacity for bile ductular transdifferentiation, but whether and how hepatocytes contribute to ductular reaction in chronic liver diseases has not been elucidated. We examined whether mouse hepatocytes can transdifferentiate into bile ductular cells in vitro, using a three-dimensional collagen gel culture method, and in vivo, using a liver repopulation model in which β-galactosidase-positive hepatocytes from Alb-Cre × ROSA26R mice were transplanted into the liver of wild-type mice. We further examined the relative contribution of intrinsic hepatocytes in ductular reaction in a hepatocyte lineage-tracing model using Mx1-Cre × ROSA26R mice treated with polyinosinic-polycytidylic acid. Within collagen gels, hepatocytes exhibited branching morphogenesis associated with the emergence of bile duct-like phenotype. In the liver repopulation model, many β-galactosidase-positive, hepatocyte-derived bile ductular structures were identified; these markedly increased after liver injury. In Mx1-Cre × ROSA26R mice, relatively minor but significant contributions of hepatocyte-derived bile ductules were observed in both periportal and centrilobular ductular reaction. As the centrilobular ductular reaction progressed, the portal ducts or ductules migrated toward the injured area and joined with hepatocyte-derived ductules, leaving the portal tract without biliary structures. We conclude that hepatocytes and bile ducts or ductules are important sources of ductular reaction and that the intrahepatic biliary system undergoes remarkable remodeling in response to chronic liver injury. |
| 注記 |
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内容記述タイプ |
Other |
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注記 |
著者最終原稿
CC-BY-NC-ND |
| 資源タイプ |
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内容記述タイプ |
Other |
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資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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25193593 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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851 |
6117.pdf (4.7 MB)
