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High glucose induces platelet derived growth factor-C via carbohydrate response element binding protein in glomeruｌar mesangial cells
https://asahikawa-med.repo.nii.ac.jp/records/5065
https://asahikawa-med.repo.nii.ac.jp/records/506516a556a2-fc7c-460f-a58a-da14905be38b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
5940.pdf (1.5 MB)
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| Item type | 学位論文 / Thesis or Dissertation_02(1) | |||||
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| 公開日 | 2015-01-29 | |||||
| タイトル | ||||||
| タイトル | High glucose induces platelet derived growth factor-C via carbohydrate response element binding protein in glomeruｌar mesangial cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Carbohydrate response element‐binding protein | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | diabetic nephropathy | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | mesangial cells | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | platelet‐derived growth factor‐C | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | transcription facto | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
橘内, 博哉
× 橘内, 博哉 |
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| 著者 ローマ字 | ||||||
| 値 | Kitsunai, Hiroya | |||||
| 書誌情報 |
Physiological Reports 巻 4, 号 6, 発行日 2016-05-01 |
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| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.14814/phy2.12730 | |||||
| 識別番号 その他 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | PMID:27033449 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲456 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2014-03-25 | |||||
| 学位名 | ||||||
| 学位名 | 博士（医学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 10107 | |||||
| 学位授与機関名 | 旭川医科大学 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Persistent high concentration of glucose causes cellular stress and damage in diabetes via derangement of gene expressions. We previously reported high glucose activates hypoxia-inducible factor-1αand downstream gene expression in mesangial cells, leading to an extracellular matrix expansion in the glomeruli. A glucose-responsive transcription factor carbohydrate response element-binding protein (ChREBP) is a key mediator for such perturbation of gene regulation. To provide insight into glucose-mediated gene regulation in mesangial cells, we performed chromatin immunoprecipitation followed byDNAmicroarray analysis and identified platelet-derived growth factor-C (PDGF-C) as a novel target gene of ChREBP In streptozotocin-induced diabetic mice, glomerular cells showed a significant increase inPDGF-C expression; the ratio ofPDGF-C-positive cells to the total number glomerular cells demonstrated more than threefold increase when compared with control animals. In cultured human mesangial cells, high glucose enhanced expression ofPDGF-C protein by 1.9-fold. Knock-down of ChREBPabrogated this induction response. UpregulatedPDGF-C contributed to the production of typeIVand typeVIcollagen, possibly via an autocrine mechanism. Interestingly, urinaryPDGF-C levels in diabetic model mice were significantly elevated in a fashion similar to urinary albumin. Taken together, we hypothesize that a high glucose-mediated induction ofPDGF-C via ChREBPin mesangial cells contributes to the development of glomerular mesangial expansion in diabetes, which may provide a platform for novel predictive and therapeutic strategies for diabetic nephropathy. | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
