| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2014-10-30 |
| タイトル |
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|
タイトル |
The involvement of myosin regulatory light chain diphosphorylation in sustained vasoconstriction under pathophysiological conditions. |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
smooth muscle |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
renal microcirculation |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
myosin phosphorylation |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
integrin-linked kinase |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
zipper-interacting protein kinase |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
竹谷, 浩介
Xuemei, Wang
Cindy, Sutherland
Iris, Kathol
Kathy, Loutzenhiser
Rodger, D. Loutzenhiser
Michael, P. Walsh
|
| 著者 ローマ字 |
|
|
|
Takeya, Kosuke |
| 書誌情報 |
Journal of Smooth Muscle Research
巻 50,
p. 18-28,
発行日 2014-01-01
|
| ISSN |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09168737 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1540/jsmr.50.18 |
| 識別番号 その他 |
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内容記述タイプ |
Other |
|
内容記述 |
PMID:24770446 |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Smooth muscle contraction is activated primarily by phosphorylation at Ser19 of the regulatory light chain subunits (LC20) of myosin II, catalysed by Ca(2+)/calmodulin-dependent myosin light chain kinase. Ca(2+)-independent contraction can be induced by inhibition of myosin light chain phosphatase, which correlates with diphosphorylation of LC20 at Ser19 and Thr18, catalysed by integrin-linked kinase (ILK) and zipper-interacting protein kinase (ZIPK). LC20 diphosphorylation at Ser19 and Thr18 has been detected in mammalian vascular smooth muscle tissues in response to specific contractile stimuli (e.g. endothelin-1 stimulation of rat renal afferent arterioles) and in pathophysiological situations associated with hypercontractility (e.g. cerebral vasospasm following subarachnoid hemorrhage). Comparison of the effects of LC 20 monophosphorylation at Ser19 and diphosphorylation at Ser19 and Thr18 on contraction and relaxation of Triton-skinned rat caudal arterial smooth muscle revealed that phosphorylation at Thr18 has no effect on steady-state force induced by Ser19 phosphorylation. On the other hand, the rates of dephosphorylation and relaxation are significantly slower following diphosphorylation at Thr18 and Ser19 compared to monophosphorylation at Ser19. We propose that this diphosphorylation mechanism underlies the prolonged contractile response of particular vascular smooth muscle tissues to specific stimuli, e.g. endothelin-1 stimulation of renal afferent arterioles, and the vasospastic behavior observed in pathological conditions such as cerebral vasospasm following subarachnoid hemorrhage and coronary arterial vasospasm. ILK and ZIPK may, therefore, be useful therapeutic targets for the treatment of such conditions. |
| 注記 |
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内容記述タイプ |
Other |
|
注記 |
publisher |
| 資源タイプ |
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内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
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|
出版タイプ |
VoR |
| フォーマット |
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内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| ID(XooNIps) |
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|
24770446 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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|
1014 |
5891.pdf (4.0 MB)
