| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2013-07-19 |
| タイトル |
|
|
タイトル |
Echinococcus multilocularis: identification and functional characterization of cathepsin B-like peptidases from metacestode |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Cestode |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Echinoccus multilocularis |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Metacestode |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Cathepsin B-like peptidase |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Recombinant enzyme |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Host proteins |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
迫, 康仁
Nakaya, Kazuhiro
伊藤, 亮
|
| 著者 ローマ字 |
|
|
|
Ito, Akira |
| 著者 ローマ字 |
|
|
|
en |
| 書誌情報 |
Experimental parasitology
巻 127,
号 3,
p. 693-701,
発行日 2011-03-01
|
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0014-4894 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.exppara.2010.11.005 |
| PubMed番号 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
21095185 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Cysteine peptidases have potent activities in the pathogenesis of various parasitic infections, and are considered as targets for chemotherapy and antigens for vaccine. In this study, two cathepsin B-like cysteine peptidases (EmCBP1 and EmCBP2) from Echinococcus multilocularis metacestodes were identified and characterized. Immunoblot analyses demonstrated that EmCBP1 and EmCBP2 were present in excretory/secretory products and extracts of E. multilocularis metacestodes. By immunohistochemistry, EmCBP1 and EmCBP2 were shown to localize to the germinal layer, the brood capsule and the protoscolex. Recombinant EmCBP1 and EmCBP2 expressed in Pichia pastoris, at optimum pH 5.5, exhibited substrate preferences for Z-Phe-Arg-MCA, Z-Val-Val-Arg-MCA, and Z-Leu-Arg-MCA, and low levels of hydrolysis of Z-Arg-Arg-MCA. Furthermore, recombinant enzymes degraded IgG, albumin, type I and IV collagens, and fibronectin. These results suggested that EmCBP1 and EmCBP2 may play key roles in protein digestion for parasites' nutrition and in parasite-host interactions. |
|
言語 |
en |
| 注記 |
|
|
内容記述タイプ |
Other |
|
注記 |
Publisher |
| 資源タイプ |
|
|
内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| ID(XooNIps) |
|
|
|
21095185 |
| 閲覧数(XooNIps) |
|
| ダウンロード数(XooNIps) |
|
|
|
993 |
5341.pdf (2.5 MB)
