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Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF MS)2機種による血液培養分離菌の同定成績について
https://asahikawa-med.repo.nii.ac.jp/records/4530
https://asahikawa-med.repo.nii.ac.jp/records/4530e241b22b-d08d-4dbe-b67d-72de13068032
名前 / ファイル | ライセンス | アクション |
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5320.pdf (378.8 kB)
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Item type | 学術雑誌論文 / Journal Article_02(1) | |||||
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公開日 | 2013-07-08 | |||||
タイトル | ||||||
タイトル | Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF MS)2機種による血液培養分離菌の同定成績について | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
キーワード | matrix-assisted laser desorption/ionization time-of-flight mass spectrometrey:MALDI-TOF MS(マトリックス支援レーザー脱離イオン化飛行時間型質量分析計) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
キーワード | blood culture(血液培養) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
キーワード | identification(同定) | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
著者 |
伊藤, 栄祐
× 伊藤, 栄祐× 渡, 智久× 東, 由桂× 渡辺, 直樹× 友田, 豊× 赤坂, 和美× 紀野, 修一 |
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著者 ローマ字 | ||||||
Ito, Eisuke | ||||||
著者 ローマ字 | ||||||
Watari, Tomohisa | ||||||
著者 ローマ字 | ||||||
Azuma, Yuka | ||||||
著者 ローマ字 | ||||||
Watanabe, Naoki | ||||||
著者 ローマ字 | ||||||
Tomoda, Yutaka | ||||||
著者 ローマ字 | ||||||
Akasaka, Kazumi | ||||||
著者 ローマ字 | ||||||
Kino, Shuichi | ||||||
書誌情報 |
臨床病理 巻 61, 号 5, p. 382-389, 発行日 2013-05-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0047-1860 | |||||
医中誌ID | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | ICHUSHI | |||||
関連識別子 | 2013301911 | |||||
識別番号 その他 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PMID:23947175 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We compared the results of two bacterial identification methods: 1)a traditional method based on phenotypic identification of the causative organism using gram-staining, culture and biochemical markers and 2)matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 111 isolates, including 107 strains of common bacteria species and 4 strains of 3 yeast species, were tested by the traditional method and MALDI-TOF MS method (VITEK MS and Micro flex LT). Data obtained using MALDI-TOF MS were classified as Level 1 and Level 2 according to the confidence level of identification results from the VITEK MS ver. 1.0 database (VITEK MS) and MALDI Biotyper ver. 2.0 database (Microflex LT). The proportions of measured samples identified as Level 1 were 98.2% with the VITEK MS database and 87.4% with the MALDI Biotyper database. The concordance rates of the traditional method were 93.7% with the VITEK MS database and 82.0% with the MALDI Biotyper database. Identification results of five strains were mismatched between the traditional method and MALDI-TOF MS. Their ribosomal RNA sequences were identical to the results obtained from MALDI-TOF MS. We concluded that the performance of VITEK MS is superior to that of the traditional method and Microflex LT. |
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注記 | ||||||
内容記述タイプ | Other | |||||
注記 | 著者最終原稿版 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
資源タイプ | text | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
ID(XooNIps) | ||||||
RB61-5-382 | ||||||
閲覧数(XooNIps) | ||||||
770 | ||||||
ダウンロード数(XooNIps) | ||||||
2710 |