| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2013-02-28 |
| タイトル |
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|
タイトル |
A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially fused mouse oocytes |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
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主題Scheme |
Other |
|
キーワード |
Chromosomal analysis |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Fused oocytes |
| キーワード |
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|
主題Scheme |
Other |
|
キーワード |
Intracytoplasmic sperm injection |
| キーワード |
|
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主題Scheme |
Other |
|
キーワード |
Livestock spermatozoa |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
渡部, 浩之
Suzuki, Hiroshi
Tateno, Hiroyuki
Fukui, Yutaka
|
| 書誌情報 |
Journal of Assisted Reproduction and Genetics
巻 27,
号 9-10,
p. 581-588,
発行日 2010-09-01
|
| ISSN |
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収録物識別子タイプ |
PISSN |
|
収録物識別子 |
1058-0468 |
| DOI |
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|
関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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|
関連識別子 |
10.1007/s10815-010-9445-0 |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Purpose: The aim of the present study was to investigate the effect of mouse oocyte volume on the efficiency of chromosomal analysis in livestock spermatozoa. \nMethods: Oocytes were injected with bull, ram, boar and dog sperm heads, and then fused with enucleated mouse oocytes. \nResults: The increment of oocyte volume increased the rates of morphologically normal oocytes after sperm injection, which induced much higher rates of overall chromosome detection in bull, ram and dog spermatozoa. The recipient oocyte volume did not affect the chromosomal integrity. Furthermore, in bull, the chromosomal integrity detected by fused mouse oocytes was similar to that derived from a homologous system. On the other hand, chromosomal plates of boar spermatozoa could not be detected despite the use of fused oocytes. \nConclusion: These data indicate that fused mouse oocytes improved the efficiency of chromosome detection in bull, ram and dog spermatozoa. |
|
言語 |
en |
| 注記 |
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内容記述タイプ |
Other |
|
注記 |
Author |
| 資源タイプ |
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|
内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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20521093 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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973 |
5073.pdf (702.1 kB)
