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Characterization of the interaction between diferric transferrin and transferrin receptor 2 by functional assays and atomic force microscopy
https://asahikawa-med.repo.nii.ac.jp/records/4308
https://asahikawa-med.repo.nii.ac.jp/records/4308d20db72f-6e27-491a-9206-2cde706bc7e7
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
5063.pdf (2.4 MB)
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| Item type | 学術雑誌論文 / Journal Article_02(1) | |||||||||||||||||
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| 公開日 | 2013-02-25 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Characterization of the interaction between diferric transferrin and transferrin receptor 2 by functional assays and atomic force microscopy | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| キーワード | iron metabolism | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| キーワード | transferrin | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| キーワード | transferrin receptor 2 | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| キーワード | atomic force microscopy | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| キーワード | functional assay | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 著者 |
生田, 克哉
× 生田, 克哉
× Yersin, Alexandre
× Ikai, Atsushi
× Aisen, Philip
× Kohgo, Yutaka
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| 書誌情報 |
Journal of molecular biology 巻 397, 号 2, p. 375-384, 発行日 2010-03-01 |
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| 収録物識別子タイプ | PISSN | |||||||||||||||||
| 収録物識別子 | 0022-2836 | |||||||||||||||||
| DOI | ||||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | 10.1016/j.jmb.2010.01.026 | |||||||||||||||||
| PubMed番号 | ||||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||
| 関連識別子 | 20096706 | |||||||||||||||||
| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Transferrin receptor 2 (TfR2), a homologue of the classical transferrin receptor 1 (TfR1), is found in two isoforms, alpha and beta. Like TfR1, TfR2alpha is a type II membrane protein, but the beta form lacks transmembrane portions and therefore is likely to be an intracellular protein. To investigate the functional properties of TfR2alpha, we expressed the protein with FLAG tagging in transferrin-receptor-deficient Chinese hamster ovary cells. The association constant for the binding of diferric transferrin (Tf) to TfR2alpha is 5.6x10(6) M(-)(1), which is about 50 times lower than that for the binding of Tf to TfR1, with correspondingly reduced rates of iron uptake. Evidence for Tf internalization and recycling via TfR2alpha without degradation, as in the TfR1 pathway, was also found. The interaction of TfR2alpha with Tf was further investigated using atomic force microscopy, a powerful tool used for investigating the interaction between a ligand and its receptor at the single-molecule level on the living cell surface. Dynamic force microscopy reveals a difference in the interactions of Tf with TfR2alpha and TfR1, with Tf-TfR1 unbinding characterized by two energy barriers, while only one is present for Tf-TfR2. We speculate that this difference may reflect Tf binding to TfR2alpha by a single lobe, whereas two lobes of Tf participate in binding to TfR1. The difference in the binding properties of Tf to TfR1 and TfR2alpha may help account for the different physiological roles of the two receptors. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 注記 | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 注記 | Author | |||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||
| 資源タイプ | text | |||||||||||||||||
| 著者版フラグ | ||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||||
| ID(XooNIps) | ||||||||||||||||||
| 20096706 | ||||||||||||||||||
| 閲覧数(XooNIps) | ||||||||||||||||||
| ダウンロード数(XooNIps) | ||||||||||||||||||
| 1237 | ||||||||||||||||||
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Cite as
生田, 克哉, Yersin, Alexandre, Ikai, Atsushi, Aisen, Philip, Kohgo, Yutaka, n.d., Characterization of the interaction between diferric transferrin and transferrin receptor 2 by functional assays and atomic force microscopy: 375–384 p.
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