| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2013-02-14 |
| タイトル |
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タイトル |
Accelerated modification of the zona pellucida is the primary cause of decreased fertilizability of oocytes in the 129 inbred mouse strain |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
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主題Scheme |
Other |
|
キーワード |
129mouse |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
Fertility |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
Oocyte |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
Zona pellucida |
| キーワード |
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主題Scheme |
Other |
|
キーワード |
Cortical granules |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
日野, 敏昭
Oda, Kanako
Nakamura, Kenji
Tateno, Hiroyuki
Toyoda, Yutaka
Yokoyama, Minesuke
|
| 書誌情報 |
Zygote
巻 19,
号 4,
p. 315-322,
発行日 2011-01-01
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| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0967-1994 |
| DOI |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1017/S0967199410000481 |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
We investigated whether the small litter size in the 129 inbred mouse strain results from a reduction in oocyte fertilizability. Sensitivity of the zona pellucida to α-chymotrypsin was examined for oocytes collected at 14 h (shortly after ovulation), 17 h, and 20 h after hCG injection. Passage of spermatozoa through the zona pellucida (using an in vitro fertilization (IVF) technique) and the density of cortical granules were examined for oocytes collected at 14 and 17 h after hCG injection. The capability of the oolemma to fuse with the sperm plasma membrane was also evaluated by IVF using zona-free eggs. The zona pellucida became markedly resistant to the enzyme 17 h after hCG injection. IVF rates significantly decreased at this time. In addition, there was a significant reduction in the density of cortical granules. When zona-free oocytes were inseminated, high fertilization rates were obtained at both 17 and 14 h after hCG injection. These results indicate that accelerated modification of the zona pellucida primarily causes a decreased fertilizability of oocytes in 129 mice, resulting in the low reproductive performance of this strain. |
|
言語 |
en |
| 注記 |
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|
内容記述タイプ |
Other |
|
注記 |
Author |
| 資源タイプ |
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内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
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出版タイプ |
AM |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| ID(XooNIps) |
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21205387 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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|
531 |
5043.pdf (319.3 kB)
