| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2013-02-14 |
| タイトル |
|
|
タイトル |
Chromosomal stability of second polar bodies in mouse embryos |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Second polar bodies |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Chromosomal stability |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Mixoploidy |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
Preimplantation genetic diagnosis |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
日野, 敏昭
日下部, 博一
立野, 裕幸
|
| 著者 ローマ字 |
|
|
|
Kusakabe, Hirokazu |
| 著者 ローマ字 |
|
|
|
en |
|
|
Tateno, Hiroyuki |
| 著者 ローマ字 |
|
|
|
en |
| 書誌情報 |
Journal of Assisted Reproduction and Genetics
巻 30,
号 1,
p. 91-98,
発行日 2012-01-01
|
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
1058-0468 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1007/s10815-012-9899-3 |
| PubMed番号 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
23224636 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
PURPOSE: Incorporation of a second polar body (PB2) into one of the blastomeres has been considered as a causal mechanism underlying diploid/triploid mixoploidy in humans. Using a mouse model, we examined whether PB2s can participate in the formation of mixoploidy. \nMETHODS: Uptake of BrdU was examined to determine DNA synthesis in PB2s up to 28 h after fertilization. PB2s from embryos at 4-6 (1-cell), 24 (2-cell), 48 (4-cell), and 72 h (morula) were fused with MII oocytes to induce premature chromosome condensation. Caspase and TUNEL assays were used to detect apoptotic PB2s at 24, 48, and 72 h. PB2s were fused with one of the blastomeres of the 2-cell embryos to produce mixoploid embryos. \nRESULTS: DNA synthesis in the PB2s continued until 22 h after fertilization. At 4-6 h, nearly all of the PB2s showed G1-type chromosomes and there was no significant increase in chromosome damage. At 24, 48, and 72 h, S-type chromatin predominated. Few PB2s showed apoptotic response until 72 h. Regardless of the fusion with the PB2, more than 90 % of the embryos developed to 4-cell stage, and over 80 % of the resultant 4-cell embryos had daughter blastomeres with a morphologically normal nucleus. Some of the daughter blastomeres displayed triploidy. \nCONCLUSIONS: The PB2 is viable for at least 72 h after fertilization, with slow progression through the cell cycle. Once the PB2 has been incorporated into a blastomere, the cell cycle of the PB2 might be synchronized with that of the host resulting in diploid/triploid mixoploidy. |
|
言語 |
en |
| 注記 |
|
|
内容記述タイプ |
Other |
|
注記 |
Author |
| 資源タイプ |
|
|
内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
|
|
出版タイプ |
AM |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| ID(XooNIps) |
|
|
|
23224636 |
| 閲覧数(XooNIps) |
|
| ダウンロード数(XooNIps) |
|
|
|
432 |
5042.pdf (365.0 kB)
