| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2013-02-14 |
| タイトル |
|
|
タイトル |
Validity of a simple vitrification technique for chromosome study of mouse one-cell embryos |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
cryopreservation |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
vitrification |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
chromosome analysis |
| キーワード |
|
|
主題Scheme |
Other |
|
キーワード |
mouse embryos |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
日野, 敏昭
日下部, 博一
立野, 裕幸
|
| 著者 ローマ字 |
|
|
|
Kusakabe, Hirokazu |
| 著者 ローマ字 |
|
|
|
en |
|
|
Tateno, Hiroyuki |
| 著者 ローマ字 |
|
|
|
en |
| 書誌情報 |
Chromosome Science
巻 13,
号 3,
p. 45-48,
発行日 2010-08-01
|
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
1344-1051 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
A total of 111 horticultural cultivars of Farfugium japonicum were studied cytologically and described morphologically for the first time. All 111 cultivars with various unusual morphologies, such as dwarf, rumpled leaves, etc., had 2n=60 chromosomes, which was the same number as in wild plants. Only one individual obtained by tissue culture of the cultivar ‘Yukibeni-botan’ had exceptional chromosome number with 2n=59. In 14 cultivars with 2n=60 chromosomes examined, no abnormal chromosome behavior was observed through the microsporogenesis, and at the meiotic metaphase I, 30 bivalents were observed. Among the flowered 96 individuals in 95 cultivars, seven individuals had no pollen grains, and those having atrophied head, such as “SHISHI” and “KURUMA”, showed relatively low pollen stainabilities, 48.3 and 23.4% in average, respectively, whereas the other cultivars had high stainability (91.3% in average). The 2n=59 somaclonal variant of the cultivar ‘Yukibeni-botan’ showed the lowest stainability (0.3%). These results indicate that horticultural cultivars of F. japonicum are stable diploids from the cytogenetical viewpoint, and that the morphological diversity in the cultivars should not be ascribed to the numerical changes of chromosomes but must have been caused by mutation of gene(s) responsible for morphogenesis. |
|
言語 |
en |
| 注記 |
|
|
内容記述タイプ |
Other |
|
注記 |
Publisher |
| 資源タイプ |
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|
内容記述タイプ |
Other |
|
資源タイプ |
text |
| 著者版フラグ |
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|
出版タイプ |
VoR |
| フォーマット |
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|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| ID(XooNIps) |
|
|
|
CS13-3-45 |
| 閲覧数(XooNIps) |
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| ダウンロード数(XooNIps) |
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|
|
246 |
5041.pdf (5.5 MB)
